PMID- 16756618
OWN - NLM
STAT- MEDLINE
DCOM- 20060724
LR  - 20161124
IS  - 0161-3499 (Print)
IS  - 0161-3499 (Linking)
VI  - 35
IP  - 4
DP  - 2006 Jun
TI  - Proinflammatory cytokine activities, matrix metalloproteinase-3 activity, and 
      sulfated glycosaminoglycan content in synovial fluid of dogs with naturally 
      acquired cranial cruciate ligament rupture.
PG  - 369-76
AB  - OBJECTIVE: To measure and compare activities of interleukin-1beta (IL-1beta), 
      interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), and matrix 
      metalloproteinase-3 (MMP-3); as well as sulfated glycosaminoglycan (S-GAG) 
      content in synovial fluid from dogs with cranial cruciate ligament rupture (CCLR) 
      and dogs with clinically normal stifles. To determine whether correlations exist 
      between demographic and disease-related variables and these synovial markers. 
      STUDY DESIGN: Prospective clinical study. ANIMALS: Dogs with CCLR (n=23) and 
      Beagles with normal stifle joints (n=21). METHODS: Synovial fluid activities of 
      proinflammatory cytokines (IL-1beta, IL-6, and TNF-alpha) were determined by 
      bioassay. MMP-3 activity was measured using fluorogenic substrate. S-GAG contents 
      were determined by dimethylmethylene blue dye-binding assay. Mann-Whitney U-test 
      was used to compare results from CCLR joints with normal controls. Spearman's 
      rank correlation test was used to evaluate associations between demographic and 
      disease-related markers and synovial markers. RESULTS: Mean values for synovial 
      markers were significantly higher in CCLR joints compared with controls. IL-1beta 
      and MMP-3 were positively correlated with lameness duration. CONCLUSIONS: 
      Activities of proinflammatory cytokines, MMP-3 activity and S-GAG contents were 
      significantly elevated in synovial fluid from canine stifle joints with naturally 
      acquired CCLR. These results indicate that there is joint inflammation and 
      increased release of GAGs into synovial fluid, suggesting that these inflammatory 
      changes are associated with depletion of proteoglycan from articular cartilage. 
      CLINICAL RELEVANCE: Medical and surgical treatments designed to decrease joint 
      inflammation and breakdown of proteoglycans may be of value in the management of 
      CCLR in the dog.
FAU - Fujita, Yukihiro
AU  - Fujita Y
AD  - Division of Veterinary Surgery, Department of Veterinary Science, Faculty of 
      Veterinary Medicine, Nippon Veterinary and Life Science University, Tokyo, Japan. 
      y-fujita@zb3.so-net.ne.jp
FAU - Hara, Yasushi
AU  - Hara Y
FAU - Nezu, Yoshinori
AU  - Nezu Y
FAU - Schulz, Kurt S
AU  - Schulz KS
FAU - Tagawa, Masahiro
AU  - Tagawa M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Vet Surg
JT  - Veterinary surgery : VS
JID - 8113214
RN  - 0 (Biomarkers)
RN  - 0 (Cytokines)
RN  - 0 (Glycosaminoglycans)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - *Anterior Cruciate Ligament Injuries
MH  - Biomarkers/metabolism
MH  - Case-Control Studies
MH  - Cytokines/metabolism
MH  - Dogs/*injuries/surgery
MH  - Female
MH  - Glycosaminoglycans/metabolism
MH  - Injury Severity Score
MH  - Interleukin-1/metabolism
MH  - Interleukin-6/metabolism
MH  - Knee Injuries/diagnostic imaging/metabolism/surgery/*veterinary
MH  - Male
MH  - Matrix Metalloproteinase 3/metabolism
MH  - Prospective Studies
MH  - Radiography
MH  - Synovial Fluid/*metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2006/06/08 09:00
MHDA- 2006/07/25 09:00
CRDT- 2006/06/08 09:00
PHST- 2006/06/08 09:00 [pubmed]
PHST- 2006/07/25 09:00 [medline]
PHST- 2006/06/08 09:00 [entrez]
AID - VSU00159 [pii]
AID - 10.1111/j.1532-950X.2006.00159.x [doi]
PST - ppublish
SO  - Vet Surg. 2006 Jun;35(4):369-76. doi: 10.1111/j.1532-950X.2006.00159.x.