PMID- 16758369
OWN - NLM
STAT- MEDLINE
DCOM- 20071025
LR  - 20181113
IS  - 1436-2228 (Print)
IS  - 1436-2228 (Linking)
VI  - 8
IP  - 4
DP  - 2006 Jul-Aug
TI  - Monitoring microbial community composition by fluorescence in situ hybridization 
      during cultivation of the marine cold-water sponge Geodia barretti.
PG  - 373-9
AB  - To determine the stability and specificity of microbes associated with the marine 
      cold-water sponge Geodia barretti during cultivation, we compared the microbial 
      community of freshly retrieved specimens to that of cultivated explants by 
      fluorescence in situ hybridization (FISH). G. barretti hosts a specific 
      homogeneous microbial community in its mesohyl, which is maintained during a 
      cultivation period of 8 months. In 10-day-old explants, bright colonies of 
      unusually large bacterial cells, located predominantly at canal walls, were 
      observed in addition to the common bacteria. Bacteria of the aberrant type 
      included both lineages present in whole sponges and foreign ones, notably 
      numerous genera of sulfate-reducing bacteria. We assume that these represent 
      infectious bacteria that eluded the innate immune system of the sponge. Explants 
      that resist these microbial attacks during the critical phase of cultivation 
      eliminate infectious bacteria. The intrinsic microbial community of G. barretti 
      is not affected by these infections and remains persistent over a cultivation 
      period of at least several months.
FAU - Hoffmann, Friederike
AU  - Hoffmann F
AD  - Max Planck Institute for Marine Microbiology, Celsiusstr. 1, D-28359, Bremen, 
      Germany. fhoffman@mpi-bremen.de
FAU - Rapp, Hans Tore
AU  - Rapp HT
FAU - Reitner, Joachim
AU  - Reitner J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060612
PL  - United States
TA  - Mar Biotechnol (NY)
JT  - Marine biotechnology (New York, N.Y.)
JID - 100892712
RN  - 0 (DNA Primers)
SB  - IM
MH  - Animals
MH  - Aquaculture/methods
MH  - Archaea/classification
MH  - Biodiversity
MH  - Cold Climate
MH  - DNA Primers/chemistry
MH  - Environmental Monitoring/*methods
MH  - Geodia/*microbiology
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Proteobacteria/classification
MH  - Seawater/*microbiology
EDAT- 2006/06/08 09:00
MHDA- 2007/10/27 09:00
CRDT- 2006/06/08 09:00
PHST- 2005/07/20 00:00 [received]
PHST- 2005/12/14 00:00 [accepted]
PHST- 2006/06/08 09:00 [pubmed]
PHST- 2007/10/27 09:00 [medline]
PHST- 2006/06/08 09:00 [entrez]
AID - 10.1007/s10126-006-5152-3 [doi]
PST - ppublish
SO  - Mar Biotechnol (NY). 2006 Jul-Aug;8(4):373-9. doi: 10.1007/s10126-006-5152-3. 
      Epub 2006 Jun 12.