PMID- 16760123
OWN - NLM
STAT- MEDLINE
DCOM- 20070112
LR  - 20060608
IS  - 1052-0295 (Print)
IS  - 1052-0295 (Linking)
VI  - 81
IP  - 1
DP  - 2006 Jan-Feb
TI  - CPD staining: an effective technique for detection of NORs and other GC-rich 
      chromosomal regions in plants.
PG  - 13-21
AB  - Mitotic chromosome spreads of 16 plant species belonging to six families were 
      analyzed using an improved combined PI and DAPI (CPD) staining procedure. 
      Fluorescence in situ hybridization (FISH) with 45S rDNA probe was conducted 
      sequentially on the same spreads to evaluate the efficiency and sensitivity of 
      the technique. Fluorochrome staining with chromomycin A3 (CMA)-DAPI also was 
      conducted to clarify the properties of the sequences involved in the CPD banded 
      regions. Our results revealed that all of the NORs (rDNA sites) in the species 
      tested were efficiently shown as red bands by CPD staining, and the number and 
      position of the bands corresponded precisely to those of the 45S rDNA FISH 
      signals, indicating that the detection sensitivity of CPD staining is similar to 
      that of FISH. In 10 of the species tested including Aegilops squarrosa, Allium 
      sativum, Oryza sativum ssp. indica, Oryza officinalis, Pisum sativum, Secale 
      cereale, Setaria italica, Sorghum vulgare, Vicia faba and Zea mays, CPD bands 
      were exhibited exclusively in their NORs, while in other six species including 
      Hordeum vulgare, Allium cepa, Psophocarpus tetragonolobus, Arabidopsis thaliana, 
      Brassica oleracea var. capitata and Lycopersicon esculentum, CPD bands appeared 
      in chromosomal regions other than their NORs. The CPD bands were in accordance 
      with the CMA bands in all species tested, indicating GC-rich sequences in the CPD 
      bands and that the improved CPD staining procedure is specific for GC-rich 
      regions in plant genomes. Our investigation not only elucidated the banding 
      mechanisms of CPD, but also demonstrated that the CPD staining technique, which 
      may be preferable to CMA staining, is an effective tool for detecting NORs and 
      other GC-rich chromosomal regions in plants.
FAU - She, C W
AU  - She CW
AD  - Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, 
      Wuhan University, Wuhan, 430072, China.
FAU - Liu, J Y
AU  - Liu JY
FAU - Song, Y C
AU  - Song YC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biotech Histochem
JT  - Biotechnic & histochemistry : official publication of the Biological Stain 
      Commission
JID - 9107378
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Indoles)
RN  - 47165-04-8 (DAPI)
SB  - IM
MH  - Chromosome Banding/*methods
MH  - Chromosomes/*chemistry/ultrastructure
MH  - *Fluorescent Dyes
MH  - *GC Rich Sequence
MH  - *Indoles
MH  - Nucleolus Organizer Region/*chemistry/ultrastructure
MH  - Plants/genetics/ultrastructure
MH  - Sensitivity and Specificity
MH  - Staining and Labeling
EDAT- 2006/06/09 09:00
MHDA- 2007/01/16 09:00
CRDT- 2006/06/09 09:00
PHST- 2006/06/09 09:00 [pubmed]
PHST- 2007/01/16 09:00 [medline]
PHST- 2006/06/09 09:00 [entrez]
AID - R543K5677PP3183J [pii]
AID - 10.1080/10520290600661414 [doi]
PST - ppublish
SO  - Biotech Histochem. 2006 Jan-Feb;81(1):13-21. doi: 10.1080/10520290600661414.