PMID- 16760404
OWN - NLM
STAT- MEDLINE
DCOM- 20060811
LR  - 20220129
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 87
IP  - Pt 7
DP  - 2006 Jul
TI  - A mouse model of persistent brain infection with recombinant Measles virus.
PG  - 2011-2019
LID - 10.1099/vir.0.81838-0 [doi]
AB  - Measles virus (MV) nucleocapsids are present abundantly in brain cells of 
      patients with subacute sclerosing panencephalitis (SSPE). This invariably lethal 
      brain disease develops years after acute measles as result of a persistent MV 
      infection. Various rodent models for MV infection of the central nervous system 
      (CNS) have been described in the past, in which the detection of viral antigens 
      is based on histological staining procedures of paraffin embedded brains. Here, 
      the usage of a recombinant MV (MV-EGFP-CAMH) expressing the haemagglutinin (H) of 
      the rodent-adapted MV-strain CAM/RB and the enhanced green fluorescent protein 
      (EGFP) is described. In newborn rodents the virus infects neurons and causes an 
      acute lethal encephalitis. From 2 weeks on, when the immune system of the 
      genetically unmodified animal is maturating, intracerebral (i.c.) infection is 
      overcome subclinically, however, a focal persistent infection in groups of 
      neurons remains. The complete brain can be analysed in 50 or 100 microm slices, 
      and infected autofluorescent cells are readily detected. Seven and 28 days 
      post-infection (p.i.) 86 and 81% of mice are infected, respectively, and virus 
      persists for more than 50 days p.i. Intraperitoneal immunization with MV 1 week 
      before infection, but not after infection, protects and prevents persistence. The 
      high percentage of persistence demonstrates that this is a reliable and useful 
      model of a persistent CNS infection in fully immunocompetent mice, which allows 
      the investigation of determinants of the immune system.
FAU - Schubert, S
AU  - Schubert S
AD  - Institute for Virology and Immunobiology, University of Wurzburg, Versbacher 
      Strasse 7, D-97078 Wurzburg, Germany.
FAU - Moller-Ehrlich, K
AU  - Moller-Ehrlich K
AD  - Institute for Virology and Immunobiology, University of Wurzburg, Versbacher 
      Strasse 7, D-97078 Wurzburg, Germany.
FAU - Singethan, K
AU  - Singethan K
AD  - Institute for Virology and Immunobiology, University of Wurzburg, Versbacher 
      Strasse 7, D-97078 Wurzburg, Germany.
FAU - Wiese, S
AU  - Wiese S
AD  - Institute for Clinical Neurobiology, University of Wurzburg, D-97078 Wurzburg, 
      Germany.
FAU - Duprex, W P
AU  - Duprex WP
AD  - School of Biomedical Sciences, The Queen's University of Belfast, Belfast BT9 
      7BL, UK.
FAU - Rima, B K
AU  - Rima BK
AD  - School of Biomedical Sciences, The Queen's University of Belfast, Belfast BT9 
      7BL, UK.
FAU - Niewiesk, S
AU  - Niewiesk S
AD  - College of Veterinary Medicine, The Ohio State University, Columbus, OH 
      43210-1093, USA.
FAU - Schneider-Schaulies, J
AU  - Schneider-Schaulies J
AD  - Institute for Virology and Immunobiology, University of Wurzburg, Versbacher 
      Strasse 7, D-97078 Wurzburg, Germany.
LA  - eng
GR  - G9901004/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Hemagglutinins, Viral)
RN  - 0 (Recombinant Proteins)
RN  - 0 (enhanced green fluorescent protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Animals, Newborn
MH  - Brain/immunology/pathology/virology
MH  - Disease Models, Animal
MH  - Green Fluorescent Proteins/genetics
MH  - Hemagglutinins, Viral/genetics
MH  - Humans
MH  - Immunization
MH  - Immunocompetence
MH  - Measles/*etiology/immunology/pathology/virology
MH  - Measles virus/*genetics/*pathogenicity
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Neurons/virology
MH  - Recombinant Proteins/genetics
MH  - Recombination, Genetic
MH  - Subacute Sclerosing Panencephalitis/*etiology/immunology/pathology/virology
MH  - T-Lymphocytes/pathology
EDAT- 2006/06/09 09:00
MHDA- 2006/08/12 09:00
CRDT- 2006/06/09 09:00
PHST- 2006/06/09 09:00 [pubmed]
PHST- 2006/08/12 09:00 [medline]
PHST- 2006/06/09 09:00 [entrez]
AID - 10.1099/vir.0.81838-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2006 Jul;87(Pt 7):2011-2019. doi: 10.1099/vir.0.81838-0.