PMID- 16761244
OWN - NLM
STAT- MEDLINE
DCOM- 20060807
LR  - 20190917
IS  - 1120-6721 (Print)
IS  - 1120-6721 (Linking)
VI  - 16
IP  - 3
DP  - 2006 May-Jun
TI  - In vivo detection of monosomy 3 in eyes with medium-sized uveal melanoma using 
      transscleral fine needle aspiration biopsy.
PG  - 422-5
AB  - PURPOSE: Cytogenetic prognostication of choroidal melanoma, particularly monosomy 
      3 detections, is limited to enucleated eyes or resected tumors. The authors 
      developed an in vivo technique to detect monosomy 3 using transscleral fine 
      needle aspiration biopsy (FNAB). METHODS: Eight eyes with medium-sized choroidal 
      melanoma were included in this prospective study. A 25-gauge transscleral FNAB 
      was performed during surgical procedure for brachytherapy, just before applying 
      the radioactive plaque over the tumor base. Sampled material underwent 
      fluorescence in situ hybridization (FISH) with centromeric probes for chromosome 
      3. Follow-up was >12 months. RESULTS: Transscleral FNAB yielded sufficient 
      material in 7 of 8 eyes (87.5 %). Five of seven eyes had monosomy 3. No early or 
      late complications were detected. CONCLUSIONS: This study demonstrates that 
      medium choroidal melanomas may be safely sampled by intraoperative transscleral 
      FNAB to detect monosomy 3 in vivo.
FAU - Midena, E
AU  - Midena E
AD  - Department of Ophthalmology, University of Padova, Padova and G. Bietti Eye 
      Foundation, IRCCS, Roma--Italy. edoardo.midena@unipd.it
FAU - Bonaldi, L
AU  - Bonaldi L
FAU - Parrozzani, R
AU  - Parrozzani R
FAU - Tebaldi, E
AU  - Tebaldi E
FAU - Boccassini, B
AU  - Boccassini B
FAU - Vujosevic, S
AU  - Vujosevic S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Eur J Ophthalmol
JT  - European journal of ophthalmology
JID - 9110772
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Biopsy, Needle
MH  - Brachytherapy
MH  - Choroid Neoplasms/*genetics/pathology/radiotherapy
MH  - Chromosomes, Human, Pair 3/*genetics
MH  - Cytogenetic Analysis
MH  - Feasibility Studies
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Melanoma/*genetics/pathology/radiotherapy
MH  - Middle Aged
MH  - *Monosomy
MH  - Prospective Studies
MH  - *Sclera
EDAT- 2006/06/09 09:00
MHDA- 2006/08/08 09:00
CRDT- 2006/06/09 09:00
PHST- 2006/06/09 09:00 [pubmed]
PHST- 2006/08/08 09:00 [medline]
PHST- 2006/06/09 09:00 [entrez]
AID - 10.1177/112067210601600310 [doi]
PST - ppublish
SO  - Eur J Ophthalmol. 2006 May-Jun;16(3):422-5. doi: 10.1177/112067210601600310.