PMID- 16762541
OWN - NLM
STAT- MEDLINE
DCOM- 20060911
LR  - 20141120
IS  - 0959-8049 (Print)
IS  - 0959-8049 (Linking)
VI  - 42
IP  - 10
DP  - 2006 Jul
TI  - Analysis of aneusomy level and HER-2 gene copy number and their effect on 
      amplification rate in breast cancer specimens read as 2+ in immunohistochemical 
      analysis.
PG  - 1501-6
AB  - Our aim was to determine the aneusomy level and the HER-2 gene copy numbers, by 
      fluorescence in situ hybridization (FISH) and to analyze their impact on the 
      amplification rate in breast carcinomas considered HER-2 weakly positive cases by 
      immunohistochemistry. We evaluated 343 breast carcinomas using double colour FISH 
      (LSI Her-2/neu gene and CEP 17). Monosomy and polysomy were demonstrated in 24.2% 
      and 46.1% respectively and 101/343 (29.6%) of the specimens were amplified by 
      FISH. A statistically significant difference was observed, when we compared the 
      amplification percentage in polysomic and monosomic specimens (P<0.0001) and, 
      among polysomic specimens, when tumours were compared with HER-2 gene signals 
      number per cell between 3 and 10 and >10 respectively (P<0.0001). Logistic 
      regression analysis showed that HER-2 signals >10 and polysomy absence were 
      independently associated with amplification. Our results confirm that the 
      majority of 2+ IHC cases express the HER-2 protein without gene amplification and 
      highlight the effect of chromosome 17 aneusomy and the HER-2 gene copy number on 
      amplification.
FAU - Merola, R
AU  - Merola R
AD  - Department of Clinical Pathology (Cytogenetic Unit), Regina Elena Cancer 
      Institute, Via Elio Chianesi, 53, 00144 Rome, Italy.
FAU - Mottolese, M
AU  - Mottolese M
FAU - Orlandi, G
AU  - Orlandi G
FAU - Vico, E
AU  - Vico E
FAU - Cognetti, F
AU  - Cognetti F
FAU - Sperduti, I
AU  - Sperduti I
FAU - Fabi, A
AU  - Fabi A
FAU - Vitelli, G
AU  - Vitelli G
FAU - Cianciulli, A M
AU  - Cianciulli AM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060609
PL  - England
TA  - Eur J Cancer
JT  - European journal of cancer (Oxford, England : 1990)
JID - 9005373
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Breast Neoplasms/*genetics
MH  - *Chromosome Aberrations
MH  - Gene Amplification
MH  - Gene Dosage
MH  - *Genes, erbB-2
MH  - Humans
MH  - Immunohistochemistry
MH  - Ploidies
MH  - Receptor, ErbB-2/*genetics
MH  - Regression Analysis
EDAT- 2006/06/10 09:00
MHDA- 2006/09/12 09:00
CRDT- 2006/06/10 09:00
PHST- 2005/12/05 00:00 [received]
PHST- 2006/02/14 00:00 [revised]
PHST- 2006/03/01 00:00 [accepted]
PHST- 2006/06/10 09:00 [pubmed]
PHST- 2006/09/12 09:00 [medline]
PHST- 2006/06/10 09:00 [entrez]
AID - S0959-8049(06)00319-4 [pii]
AID - 10.1016/j.ejca.2006.03.011 [doi]
PST - ppublish
SO  - Eur J Cancer. 2006 Jul;42(10):1501-6. doi: 10.1016/j.ejca.2006.03.011. Epub 2006 
      Jun 9.