PMID- 16763200
OWN - NLM
STAT- MEDLINE
DCOM- 20061006
LR  - 20150813
IS  - 1066-5099 (Print)
IS  - 1066-5099 (Linking)
VI  - 24
IP  - 9
DP  - 2006 Sep
TI  - In vivo bone formation by human bone marrow stromal cells: reconstruction of the 
      mouse calvarium and mandible.
PG  - 2140-9
AB  - Bone marrow stromal cells (BMSCs) contain a subset of multipotent cells with the 
      potential to repair hard-tissue defects. Mouse BMSCs, combined with a collagen 
      carrier, can close critical-sized homologous mouse calvarial defects, but this 
      new bone has a poor union with the adjacent calvarium. When human BMSCs are 
      transplanted for the purpose of engineering new bone, best results can be 
      achieved if the cells are combined with hydroxyapatite/tricalcium phosphate 
      (HA/TCP) particles. Here, we demonstrate that transplantation of cultured human 
      BMSCs in conjunction with HA/TCP particles can be used successfully to close 
      mouse craniofacial bone defects and that removal of the periosteum from the 
      calvarium significantly enhances union with the transplant. Transplants were 
      followed for up to 96 weeks and were found to change in morphology but not bone 
      content after 8 weeks; this constitutes the first description of human BMSCs 
      placed long-term to heal bone defects. New bone formation continued to occur in 
      the oldest transplants, confirmed by tetracycline labeling. Additionally, the 
      elastic modulus of this engineered bone resembled that of the normal mouse 
      calvarium, and our use of atomic force microscopy (AFM)-based nanoindentation 
      offered us the first opportunity to compare these small transplants against 
      equally minute mouse bones. Our results provide insights into the long-term 
      behavior of newly engineered orthotopic bone from human cells and have powerful 
      implications for therapeutic human BMSC transplantation.
FAU - Mankani, Mahesh H
AU  - Mankani MH
AD  - Department of Surgery, Division of Plastic Surgery, University of California, San 
      Francisco, 1001 Potrero Avenue, Box 0807, 94143-0807, USA. 
      mmankani@sfghsurg.ucsf.edu
FAU - Kuznetsov, Sergei A
AU  - Kuznetsov SA
FAU - Wolfe, Raymond M
AU  - Wolfe RM
FAU - Marshall, Grayson W
AU  - Marshall GW
FAU - Robey, Pamela Gehron
AU  - Robey PG
LA  - eng
GR  - Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20060608
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology
MH  - Bone Marrow Transplantation
MH  - Cells, Cultured
MH  - Female
MH  - Humans
MH  - Mandible/*cytology
MH  - Mice
MH  - Osteogenesis/*physiology
MH  - Skull/*cytology
MH  - Stromal Cells/*cytology
MH  - Time Factors
EDAT- 2006/06/10 09:00
MHDA- 2006/10/07 09:00
CRDT- 2006/06/10 09:00
PHST- 2006/06/10 09:00 [pubmed]
PHST- 2006/10/07 09:00 [medline]
PHST- 2006/06/10 09:00 [entrez]
AID - 2005-0567 [pii]
AID - 10.1634/stemcells.2005-0567 [doi]
PST - ppublish
SO  - Stem Cells. 2006 Sep;24(9):2140-9. doi: 10.1634/stemcells.2005-0567. Epub 2006 
      Jun 8.