PMID- 1676433
OWN - NLM
STAT- MEDLINE
DCOM- 19910808
LR  - 20190827
IS  - 0165-0270 (Print)
IS  - 0165-0270 (Linking)
VI  - 36
IP  - 1
DP  - 1991 Jan
TI  - In vitro microdialysis: a novel technique for stimulated neurotransmitter release 
      measurements.
PG  - 85-90
AB  - A novel microdialysis technique suitable for parallel measurements of 
      neurotransmitter release and single unit recordings from brain slices is 
      presented. The effects of 3,4-methylenedioxymethamphetamine (MDMA) on slices of 
      dorsal raphe nucleus and frontal cortex in a perfusion chamber for 
      electrophysiological measures were studied. MDMA caused measurable release of 
      serotonin which, in the case of the dorsal raphe, was of similar duration as the 
      period of reduced cell firing induced by MDMA. Tryptophan potentiated the action 
      of MDMA. Possible additional applications of this technique are discussed.
FAU - Bradberry, C W
AU  - Bradberry CW
AD  - Dept. of Pharmacology, Yale University School of Medicine, New Haven, CT 06510.
FAU - Sprouse, J S
AU  - Sprouse JS
FAU - Sheldon, P W
AU  - Sheldon PW
FAU - Aghajanian, G K
AU  - Aghajanian GK
FAU - Roth, R H
AU  - Roth RH
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Neurosci Methods
JT  - Journal of neuroscience methods
JID - 7905558
RN  - 0 (Neurotransmitter Agents)
RN  - 333DO1RDJY (Serotonin)
RN  - 4764-17-4 (3,4-Methylenedioxyamphetamine)
RN  - 8DUH1N11BX (Tryptophan)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - 3,4-Methylenedioxyamphetamine/analogs & derivatives/pharmacology
MH  - Animals
MH  - Brain Chemistry/*drug effects
MH  - Chromatography, High Pressure Liquid
MH  - Dialysis
MH  - Electrophysiology
MH  - In Vitro Techniques
MH  - Male
MH  - Microchemistry
MH  - N-Methyl-3,4-methylenedioxyamphetamine
MH  - Neurotransmitter Agents/*metabolism
MH  - Raphe Nuclei/drug effects/metabolism
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Serotonin/metabolism
MH  - Tryptophan/metabolism
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
AID - 0165-0270(91)90141-L [pii]
AID - 10.1016/0165-0270(91)90141-l [doi]
PST - ppublish
SO  - J Neurosci Methods. 1991 Jan;36(1):85-90. doi: 10.1016/0165-0270(91)90141-l.