PMID- 16767176
OWN - NLM
STAT- MEDLINE
DCOM- 20061011
LR  - 20161124
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 49
IP  - 5
DP  - 2006 May
TI  - Variations in abundance of 2 repetitive sequences in Leymus and Psathyrostachys 
      species.
PG  - 511-9
AB  - The Ns genome of the genus Psathyrostachys is a component of the polyploid genome 
      in the genus Leymus. Using fluorescence in situ hybridization (FISH), the 
      occurrence and abundance of 2 tandem repetitive sequences from Leymus racemosus 
      (Lam.) Tzvelev, pLrTaiI-1 (TaiI family) and pLrPstI-1 (1 class of 350-bp family), 
      were assayed in 4 species of the genera Psathyrostachys and Leymus. The pLrPstI-1 
      sequence was absent in all 4 Psathyrostachys species. While P. fragilis and P. 
      huashanica did not have the pLrTaiI-1 sequence, 15 accessions of P. juncea and 2 
      accessions of P. lanuginosa had pLrTaiI-1 sites ranging in number from 7 to 16 
      and from 2 to 21, respectively. The numbers of pLrTaiI-1 and pLrPstI-1 sites were 
      1-24 and 0-30, respectively, in L. ramosus; 2-31 and 5-36 in L. racemosus; 0-4 
      and 0 in L. mollis; 2-9 and 24-27 in L. secalinus. The FISH assay on pLrTaiI-1 
      was successfully converted to a sequence-tagged-site polymerase chain reaction 
      (STS-PCR) test using a primer pair designed from the sequence of this repetitive 
      DNA. Seventy-three accessions representing 27 Leymus species were assayed for the 
      abundance of pLrTaiI-1 by STS-PCR. With a few exceptions of uniformity in some 
      accessions, nearly all Leymus species observed were heterogeneous for the 
      abundance of pLrTaiI-1 sequence and no Leymus species was totally devoid of this 
      repetitive sequence. These findings may have significance for the understanding 
      of phylogeny, nature of polyploidy, adaptive ranges, and breeding potential of 
      Leymus species.
FAU - Wang, R R-C
AU  - Wang RR
AD  - Laboratory of Plant Genetics and Breeding Science, Faculty of Agriculture, 
      Tottori University, Japan. rrcwang@cc.usu.edu
FAU - Zhang, J-Y
AU  - Zhang JY
FAU - Lee, B S
AU  - Lee BS
FAU - Jensen, K B
AU  - Jensen KB
FAU - Kishii, M
AU  - Kishii M
FAU - Tsujimoto, H
AU  - Tsujimoto H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
SB  - IM
MH  - Blotting, Southern
MH  - Chromosomes, Plant/diagnostic imaging
MH  - Cytogenetic Analysis
MH  - Elymus/*genetics
MH  - *Gene Dosage
MH  - *Genetic Variation
MH  - In Situ Hybridization, Fluorescence
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Restriction Fragment Length
MH  - Radiography
MH  - *Repetitive Sequences, Nucleic Acid
EDAT- 2006/06/13 09:00
MHDA- 2006/10/13 09:00
CRDT- 2006/06/13 09:00
PHST- 2006/06/13 09:00 [pubmed]
PHST- 2006/10/13 09:00 [medline]
PHST- 2006/06/13 09:00 [entrez]
AID - g05-126 [pii]
AID - 10.1139/g05-126 [doi]
PST - ppublish
SO  - Genome. 2006 May;49(5):511-9. doi: 10.1139/g05-126.