PMID- 16767177
OWN - NLM
STAT- MEDLINE
DCOM- 20061011
LR  - 20161020
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 49
IP  - 5
DP  - 2006 May
TI  - The clustering of four subfamilies of satellite DNA at individual chromosome ends 
      in Silene latifolia.
PG  - 520-30
AB  - The satellite DNA (satDNA) on the ends of chromosomes has been isolated and 
      characterized in the dioecious plant Silene latifolia. BAC clones containing 
      large numbers of repeat units of satDNA in a tandem array were isolated to 
      examine the clustering of the repeat units. satDNA repeat units were purified 
      from each isolated BAC clone and sequenced. To investigate pairwise similarities 
      among the repeat units, a phylogenetic tree was constructed using the 
      neighbor-joining algorithm. The repeat units derived from 7 BAC clones were 
      grouped into SacI, KpnI, #11F02, and #16E07 subfamilies. The SacI and KpnI 
      subfamilies have been reported previously. Multicolored fluorescence in situ 
      hybridization (FISH) using SacI or KpnI subfamily probes resulted in different 
      signal intensities and locations at the chromosomal ends, indicating that each 
      chromosomal end has a unique composition of subfamilies of satDNA. For example, 
      the p arm of the X chromosome exhibited signal composition similar to that on the 
      pseudo autosomal region (PAR) of the Y chromosome, but not to that on the q arm 
      of the X chromosome. The satDNA has not been completely homogenized in the S. 
      latifolia genome. Each subfamily is available for a probe of FISH karyotyping.
FAU - Kazama, Yusuke
AU  - Kazama Y
AD  - Integrated Bioscience, Graduate School of Frontier Sciences, University of Tokyo, 
      Japan. ykaze@riken.jp
FAU - Sugiyama, Ryuji
AU  - Sugiyama R
FAU - Suto, Yumiko
AU  - Suto Y
FAU - Uchida, Wakana
AU  - Uchida W
FAU - Kawano, Shigeyuki
AU  - Kawano S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (DNA, Satellite)
SB  - IM
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - Chromosome Structures/*metabolism
MH  - Chromosomes, Artificial, Bacterial/metabolism
MH  - *Chromosomes, Plant
MH  - Consensus Sequence
MH  - *DNA, Satellite
MH  - In Situ Hybridization, Fluorescence
MH  - Metaphase
MH  - Molecular Sequence Data
MH  - *Multigene Family
MH  - Phylogeny
MH  - Restriction Mapping
MH  - Sequence Homology, Nucleic Acid
MH  - Silene/*genetics
EDAT- 2006/06/13 09:00
MHDA- 2006/10/13 09:00
CRDT- 2006/06/13 09:00
PHST- 2006/06/13 09:00 [pubmed]
PHST- 2006/10/13 09:00 [medline]
PHST- 2006/06/13 09:00 [entrez]
AID - g05-130 [pii]
AID - 10.1139/g05-130 [doi]
PST - ppublish
SO  - Genome. 2006 May;49(5):520-30. doi: 10.1139/g05-130.