PMID- 16767658
OWN - NLM
STAT- MEDLINE
DCOM- 20090327
LR  - 20060612
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 23
IP  - 3
DP  - 2006 Jun
TI  - [Influence of postovulatory ageing on balanced predivision of sister chromatid in 
      mouse oocytes].
PG  - 256-9
AB  - OBJECTIVE: To study the impact of postovulatory ageing to balanced predivision of 
      oocyte sister chromatid. METHODS: The mouse oocytes were cultured 0-72 h. Then 
      chromosome 16 was detected by fluorescence in situ hybridization (FISH). The 
      oocyte spindle and chromosome configuration were examined by immunocytochemistry. 
      RESULTS: For freshly ovulated mouse oocyte, the balanced predivision of sister 
      chromatid occurred only at 7%. However, for oocytes cultured for 24 h, 48 h and 
      72 h in vitro, the balanced predivision of sister chromatid occurred up to at 
      32%, 51% or 62% respectively (P< 0.01). The abnormal cell spindle and chromosome 
      configuration occurred at 9% of freshly ovulated oocytes, but it increased to 
      63%, 83% and 98% when the oocytes were cultured in vitro for 24 h, 48 h or 72 h 
      respectively (P< 0.01). CONCLUSION: The occurrence of balanced predivision of 
      oocyte sister chromatid may result during postovulatory ageing, and may be 
      related to change of oocyte spindle and chromosome configuration.
FAU - Chen, Wen
AU  - Chen W
AD  - Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, Hubei, 430030 PR China. 
      chenwenyyy@yahoo.com
FAU - Liu, Qun
AU  - Liu Q
FAU - Li, Zhou
AU  - Li Z
FAU - Wang, Xin-rong
AU  - Wang XR
FAU - Zhu, Gui-jin
AU  - Zhu GJ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
SB  - IM
MH  - Aging/*physiology
MH  - Animals
MH  - Cells, Cultured
MH  - Chromatids/*genetics
MH  - Chromosomes, Mammalian/genetics
MH  - Female
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Mice
MH  - Oocytes/cytology/*metabolism
EDAT- 2006/06/13 09:00
MHDA- 2009/03/28 09:00
CRDT- 2006/06/13 09:00
PHST- 2006/06/13 09:00 [pubmed]
PHST- 2009/03/28 09:00 [medline]
PHST- 2006/06/13 09:00 [entrez]
AID - 940623065 [pii]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2006 Jun;23(3):256-9.