PMID- 16769584
OWN - NLM
STAT- MEDLINE
DCOM- 20061005
LR  - 20060613
IS  - 1592-8721 (Electronic)
IS  - 0390-6078 (Linking)
VI  - 91
IP  - 6
DP  - 2006 Jun
TI  - A combination of cytomorphology, cytogenetic analysis, fluorescence in situ 
      hybridization and reverse transcriptase polymerase chain reaction for 
      establishing clonality in cases of persisting hypereosinophilia.
PG  - 817-20
AB  - To evaluate the frequency of clonal abnormalities in patients with unexplained 
      persisting eosinophilia we analyzed 40 patients (27 males, 13 females) using 
      cytomorphology, cytogenetic analysis, interphase fluorescence in situ 
      hybridization (FISH), and reverse transcriptase polymerase chain reaction 
      (RT-PCR). Cytogenetic analysis revealed clonal abnormalities in five patients 
      (four of whom were males) including t(8;9)(p21;p24), ins(9;4)(q34;q12q31), 
      del(6)(q24), and trisomy 8 (n=2). RT-PCR confirmed a PCM1-JAK2 fusion underlying 
      the t(8;9). FISH analysis suggested a rearrangement involving PDGFRA in the 
      ins(9;4). A FIP1L1-PDGFRA fusion gene was identified in four male patients by 
      interphase FISH and RT-PCR. These methods in combination demonstrated clonality 
      in 8/40 patients (20%) with a male predominance (6/8; 75%).
FAU - Bacher, Ulrike
AU  - Bacher U
AD  - Department of Clinical Chemistry, Ludwig-Maximilians-University, Munich 
      Marchioninistr. 15, 81377 Munich. ulrike.bacher@med.uni-muenchen.de
FAU - Reiter, Andreas
AU  - Reiter A
FAU - Haferlach, Torsten
AU  - Haferlach T
FAU - Mueller, Lothar
AU  - Mueller L
FAU - Schnittger, Susanne
AU  - Schnittger S
FAU - Kern, Wolfgang
AU  - Kern W
FAU - Schoch, Claudia
AU  - Schoch C
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Haematologica
JT  - Haematologica
JID - 0417435
SB  - IM
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 8
MH  - Chromosomes, Human, Pair 9
MH  - Cytogenetic Analysis
MH  - Female
MH  - Gene Rearrangement
MH  - Humans
MH  - Hypereosinophilic Syndrome/*genetics/*pathology
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Retrospective Studies
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sex Characteristics
MH  - Translocation, Genetic
EDAT- 2006/06/14 09:00
MHDA- 2006/10/06 09:00
CRDT- 2006/06/14 09:00
PHST- 2006/06/14 09:00 [pubmed]
PHST- 2006/10/06 09:00 [medline]
PHST- 2006/06/14 09:00 [entrez]
PST - ppublish
SO  - Haematologica. 2006 Jun;91(6):817-20.