PMID- 16769699
OWN - NLM
STAT- MEDLINE
DCOM- 20071012
LR  - 20090224
IS  - 1756-1663 (Electronic)
IS  - 1340-2838 (Linking)
VI  - 12
IP  - 6
DP  - 2005
TI  - Isolation and expression profiling of genes upregulated in the peripheral blood 
      cells of systemic lupus erythematosus patients.
PG  - 429-39
AB  - We have identified the genes whose expressions are augmented in the blood cells 
      of the patients with systemic lupus erythematosus (SLE) using the 'stepwise 
      subtraction' technique along with microarray analysis. The expression levels of 
      these genes were assessed by quantitative real-time reverse transcription 
      polymerase chain reaction (RT-PCR) in 31 SLE patients and 30 healthy controls. We 
      found that the transcription levels of following eight genes were significantly 
      increased in SLE patients; interferon (IFN)-alpha-inducible protein 27 (IFI27), 
      IFN-alpha-inducible protein IFI-15K (G1P2), IFN stimulated gene 20 kDa (ISG20), 
      epithelial stromal interaction 1 (EPSTI1), defensin-alpha (DEFA3), amphiregulin 
      (AREG) and two genes of unknown function (BLAST accession nos AL050290 and 
      AY358224 = SLED1). In comparison with idiopathic thrombocytopenic purpura (ITP), 
      an organ-specific autoimmune disease, IFI27, G1P2 and SLED1 were preferentially 
      upregulated in SLE. In contrast, AREG and AL050290 were more highly expressed in 
      ITP than in SLE. We correlated changes in gene expression and clinical/laboratory 
      features of SLE and found that expression of ISG20, EPSTI1 and SLED1 are 
      significantly correlated with lymphocyte counts. Genes linked to IFN are well 
      known to influence SLE, but several other novel genes unrelated to IFN signaling 
      we report here would be useful to understand the pathophysiology of SLE.
FAU - Ishii, Taeko
AU  - Ishii T
AD  - Department of Molecular Medicine, Graduate School of Medicine, Osaka University 
      Suita, Japan.
FAU - Onda, Hiroaki
AU  - Onda H
FAU - Tanigawa, Akie
AU  - Tanigawa A
FAU - Ohshima, Shiro
AU  - Ohshima S
FAU - Fujiwara, Hiroshi
AU  - Fujiwara H
FAU - Mima, Toru
AU  - Mima T
FAU - Katada, Yoshinori
AU  - Katada Y
FAU - Deguchi, Hitoshi
AU  - Deguchi H
FAU - Suemura, Masaki
AU  - Suemura M
FAU - Miyake, Tadao
AU  - Miyake T
FAU - Miyatake, Kunio
AU  - Miyatake K
FAU - Kawase, Ichiro
AU  - Kawase I
FAU - Zhao, Hanjun
AU  - Zhao H
FAU - Tomiyama, Yoshiaki
AU  - Tomiyama Y
FAU - Saeki, Yukihiko
AU  - Saeki Y
FAU - Nojima, Hiroshi
AU  - Nojima H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060223
PL  - England
TA  - DNA Res
JT  - DNA research : an international journal for rapid publication of reports on genes 
      and genomes
JID - 9423827
RN  - 0 (Proteome)
SB  - IM
MH  - Female
MH  - Gene Expression Profiling
MH  - Gene Library
MH  - Humans
MH  - Leukocytes, Mononuclear/*metabolism
MH  - Lupus Erythematosus, Systemic/*blood
MH  - Male
MH  - Oligonucleotide Array Sequence Analysis
MH  - Proteome/*metabolism
MH  - Purpura, Thrombocytopenic, Idiopathic/metabolism
MH  - *Up-Regulation
EDAT- 2006/06/14 09:00
MHDA- 2007/10/13 09:00
CRDT- 2006/06/14 09:00
PHST- 2006/06/14 09:00 [pubmed]
PHST- 2007/10/13 09:00 [medline]
PHST- 2006/06/14 09:00 [entrez]
AID - dsi020 [pii]
AID - 10.1093/dnares/dsi020 [doi]
PST - ppublish
SO  - DNA Res. 2005;12(6):429-39. doi: 10.1093/dnares/dsi020. Epub 2006 Feb 23.