PMID- 16771778
OWN - NLM
STAT- MEDLINE
DCOM- 20060809
LR  - 20211203
IS  - 0905-6157 (Print)
IS  - 0905-6157 (Linking)
VI  - 17
IP  - 4
DP  - 2006 Jun
TI  - Effects of leukotriene receptor antagonists on monocyte chemotaxis, p38 and 
      cytoplasmic calcium.
PG  - 250-8
AB  - Montelukast and zafirlukast, two cysteinyl leukotriene receptor antagonists 
      (LTRAs), have been shown to have a beneficial effect on the clinical symptoms of 
      asthma. LTRAs can inhibit eosinophil recruitment; however, little is known about 
      their role in monocyte migration. We investigated whether montelukast and 
      zafirlukast could suppress chemokine-induced chemotaxis of monocytes and 
      signaling. Chemotaxis of monocytes from peripheral blood mononuclear cells 
      (PBMCs), cord blood mononuclear cells (CBMCs), and THP-1 cells was evaluated 
      using a 24-well transwell microchamber. [Ca2+]i was measured with the 
      fluorescence calcium indicator fura-2/AM photometry system. p38 MAPK expression 
      was measured by Western blotting. Results showed that montelukast (1-100 microm) 
      and zafirlukast (100 microm) significantly down-regulated monocyte 
      chemoattractant protein-1(MCP-1)-induced chemotaxis of THP-1 cells and human 
      primary monocytes from PBMCs and CBMCs (p<0.05, each comparison). Montelukast 
      also abolished MCP-1-induced [Ca2+]i and pp38 MAPK expression in THP-1 cells in a 
      dose-dependent manner. These data demonstrate that montelukast is effective in 
      down-regulating human monocyte chemotaxis induced by MCP-1. This effect may 
      involve the down-regulation of MCP-1-induced [Ca2+]i and p38 MAPK expression.
FAU - Hung, Chih-Hsing
AU  - Hung CH
AD  - Department of Pediatrics, Tri-service General Hospital, National Defense Medical 
      Center, Taipei, and Department of Pediatrics, Faculty of Pediatrics, College of 
      Medicine, Kaohsiung Medical University, Taiwan, Republic of China. 
      pedhung@kmu.edu.fw
FAU - Li, Chi-Yuan
AU  - Li CY
FAU - Hua, Yi-Ming
AU  - Hua YM
FAU - Chen, Chun-Jung
AU  - Chen CJ
FAU - Yang, Kuender D
AU  - Yang KD
FAU - Jong, Yuh-Jyh
AU  - Jong YJ
LA  - eng
PT  - Journal Article
PL  - England
TA  - Pediatr Allergy Immunol
JT  - Pediatric allergy and immunology : official publication of the European Society 
      of Pediatric Allergy and Immunology
JID - 9106718
RN  - 0 (Acetates)
RN  - 0 (Anti-Asthmatic Agents)
RN  - 0 (Cyclopropanes)
RN  - 0 (Indoles)
RN  - 0 (Leukotriene Antagonists)
RN  - 0 (Phenylcarbamates)
RN  - 0 (Quinolines)
RN  - 0 (Sulfides)
RN  - 0 (Sulfonamides)
RN  - 0 (Tosyl Compounds)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - MHM278SD3E (montelukast)
RN  - SY7Q814VUP (Calcium)
RN  - XZ629S5L50 (zafirlukast)
SB  - IM
MH  - Acetates/pharmacology
MH  - Anti-Asthmatic Agents/*pharmacology
MH  - Blotting, Western
MH  - Calcium/*metabolism
MH  - Cell Separation
MH  - Chemotaxis/*drug effects
MH  - Cyclopropanes
MH  - Dose-Response Relationship, Drug
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Fetal Blood
MH  - Flow Cytometry
MH  - Humans
MH  - Indoles
MH  - Intracellular Fluid/drug effects/metabolism
MH  - Leukotriene Antagonists/*pharmacology
MH  - Monocytes/*drug effects/enzymology/metabolism
MH  - Phenylcarbamates
MH  - Photometry
MH  - Pregnancy
MH  - Quinolines/pharmacology
MH  - Reference Values
MH  - Sulfides
MH  - Sulfonamides
MH  - Time Factors
MH  - Tosyl Compounds/pharmacology
MH  - p38 Mitogen-Activated Protein Kinases/*drug effects/metabolism
EDAT- 2006/06/15 09:00
MHDA- 2006/08/10 09:00
CRDT- 2006/06/15 09:00
PHST- 2006/06/15 09:00 [pubmed]
PHST- 2006/08/10 09:00 [medline]
PHST- 2006/06/15 09:00 [entrez]
AID - PAI385 [pii]
AID - 10.1111/j.1399-3038.2006.00385.x [doi]
PST - ppublish
SO  - Pediatr Allergy Immunol. 2006 Jun;17(4):250-8. doi: 
      10.1111/j.1399-3038.2006.00385.x.