PMID- 1677645
OWN - NLM
STAT- MEDLINE
DCOM- 19910905
LR  - 20061115
IS  - 0021-9541 (Print)
IS  - 0021-9541 (Linking)
VI  - 148
IP  - 1
DP  - 1991 Jul
TI  - Kidney-specific enzyme expression by human kidney cell lines generated through 
      oncogene transfection.
PG  - 54-9
AB  - The human kidney cell line 293 was generated by transfection of adenovirus DNA 
      into normal human embryonic kidney (HEK) cells (Graham et al., 1977), whereas the 
      human kidney cell lines ST-1i and STt-4i were generated by transfection of HEK 
      cells with plasmids encoding SV40 viral oncogenes (Abcouwer et al., 1989). In 
      this study, we examined kidney-specific enzyme activity levels in 293, ST-1i, and 
      STt-4i cells to determine their ability to exhibit kidney-specific gene 
      expression. Enzymes examined were leucine aminopeptidase (LAP), gamma-glutamyl 
      transpeptidase (gamma-GTP), and the disaccharidases trehalase and maltase. 
      Enzymatic activity levels were compared to three other kidney cell lines (MDCK, 
      OK, and LLC-PK1) as well as to normal human embryonic kidney (HEK) cells and the 
      human hepatoma cell line, Hep G2. Modulation of kidney-specific enzyme activities 
      was assessed in response to several differentiation-inducing agents (adenosine, 
      n-butyric acid, hexamethylene bisacetamide (HMBA), dimethyl sulfoxide (DMSO), 
      N,N'-dimethylformamide (DMF), isobutyl methyl xanthine (IBMX), di butyryl cAMP, 
      and retinoic acid). ST-1i and STt-4i exhibit elevated levels of LAP, gamma-GTP, 
      trehalase, and maltase, consistent with their kidney cell origin, whereas 293 
      cells exhibit elevated levels of just gamma-GTP and maltase. Maltase and 
      gamma-GTP enzyme activities in ST-1i and STt-4i cells were very responsive to the 
      various inducing agents; 293 cells were less responsive at the inducer 
      concentrations examined. None of the three human cell lines formed domes under 
      any of the experimental conditions. In summary, ST-1i and STt-4i are comparable 
      to normal HEK cells in expression of kidney-specific enzymes and in 
      responsiveness to differentiation-inducing agents, in spite of continued 
      expression of SV40 oncogenes.
FAU - Robinson, P S
AU  - Robinson PS
AD  - Department of Chemical Engineering, University of Houston, Texas 77004.
FAU - Goochee, C F
AU  - Goochee CF
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (DNA, Viral)
RN  - EC 2.3.2.2 (gamma-Glutamyltransferase)
RN  - EC 3.2.1.20 (alpha-Glucosidases)
RN  - EC 3.2.1.28 (Trehalase)
RN  - EC 3.4.11.1 (Leucyl Aminopeptidase)
SB  - IM
MH  - Animals
MH  - Carcinoma, Hepatocellular/enzymology/pathology
MH  - Cell Differentiation/drug effects
MH  - Cell Line
MH  - DNA, Viral/genetics
MH  - Humans
MH  - Kidney/cytology/embryology/*enzymology
MH  - Leucyl Aminopeptidase/*genetics/metabolism
MH  - Liver Neoplasms/enzymology/pathology
MH  - Oncogenes/*genetics
MH  - Simian virus 40/genetics
MH  - Transfection/*genetics
MH  - Trehalase/*genetics/metabolism
MH  - alpha-Glucosidases/*genetics/metabolism
MH  - gamma-Glutamyltransferase/*genetics/metabolism
EDAT- 1991/07/01 00:00
MHDA- 1991/07/01 00:01
CRDT- 1991/07/01 00:00
PHST- 1991/07/01 00:00 [pubmed]
PHST- 1991/07/01 00:01 [medline]
PHST- 1991/07/01 00:00 [entrez]
AID - 10.1002/jcp.1041480107 [doi]
PST - ppublish
SO  - J Cell Physiol. 1991 Jul;148(1):54-9. doi: 10.1002/jcp.1041480107.