PMID- 16777063
OWN - NLM
STAT- MEDLINE
DCOM- 20060821
LR  - 20081121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 346
IP  - 3
DP  - 2006 Aug 4
TI  - Purification and characterization of a heat-stable serine protease inhibitor from 
      the tubers of new potato variety "Golden Valley".
PG  - 681-6
AB  - Potide-G, a small (5578.9 Da) antimicrobial peptide, was isolated from potato 
      tubers (Solanum tuberosum L. cv. Golden Valley) through extraction of the 
      water-soluble fraction, dialysis, ultrafiltration and DEAE-cellulose and C18 
      reverse-phase high performance liquid chromatography. This antimicrobial peptide 
      was heat-stable and almost completely suppressed the proteolytic activity of 
      trypsin, chymotrypsin and papain, with no hemolytic activity. In addition, 
      potide-G potently inhibited growth of a variety of bacterial (Staphylococcus 
      aureus, Listeria monocytogenes, Escherichia coli, and Clavibacter michiganense 
      subsp. michiganinse) and fungal (Candida albicans and Rhizoctonia solani) 
      strains. Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) 
      mass spectrometry revealed that the N-terminal sequence (residues from 1 to 11) 
      of the protein is identical to that of potato proteinase inhibitor, a member of 
      the Kunitz superfamily. And like other members of this class of protease 
      inhibitor, potide-G may have a number of beneficial and therapeutic uses.
FAU - Kim, Mi-Hyun
AU  - Kim MH
AD  - Research Center for Proteineous Materials, Chosun University, Kwangju 501-759, 
      Republic of Korea.
FAU - Park, Seong-Cheol
AU  - Park SC
FAU - Kim, Jin-Young
AU  - Kim JY
FAU - Lee, Sun Young
AU  - Lee SY
FAU - Lim, Hak-Tae
AU  - Lim HT
FAU - Cheong, Hyeonsook
AU  - Cheong H
FAU - Hahm, Kyung-Soo
AU  - Hahm KS
FAU - Park, Yoonkyung
AU  - Park Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060609
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Antifungal Agents)
RN  - 0 (Plant Proteins)
RN  - 0 (Serine Proteinase Inhibitors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Anti-Bacterial Agents/chemistry/isolation & purification/metabolism/pharmacology
MH  - Antifungal Agents/chemistry/isolation & purification/metabolism/pharmacology
MH  - Erythrocytes/cytology/drug effects
MH  - Hemolysis/drug effects
MH  - Hot Temperature
MH  - Humans
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Plant Proteins/*chemistry/*isolation & purification/pharmacology
MH  - Plant Tubers/*chemistry
MH  - Sequence Alignment
MH  - Serine Proteinase Inhibitors/chemistry/*isolation & purification/*pharmacology
MH  - Solanum tuberosum/*chemistry/*classification
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
EDAT- 2006/06/17 09:00
MHDA- 2006/08/22 09:00
CRDT- 2006/06/17 09:00
PHST- 2006/05/18 00:00 [received]
PHST- 2006/05/22 00:00 [accepted]
PHST- 2006/06/17 09:00 [pubmed]
PHST- 2006/08/22 09:00 [medline]
PHST- 2006/06/17 09:00 [entrez]
AID - S0006-291X(06)01201-0 [pii]
AID - 10.1016/j.bbrc.2006.05.186 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2006 Aug 4;346(3):681-6. doi: 
      10.1016/j.bbrc.2006.05.186. Epub 2006 Jun 9.