PMID- 16777066
OWN - NLM
STAT- MEDLINE
DCOM- 20060821
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 346
IP  - 3
DP  - 2006 Aug 4
TI  - Construction and characterization of a pseudo-immune human antibody library using 
      yeast surface display.
PG  - 896-903
AB  - Lymphocytes from eight individuals out of 60 healthy donors, whose plasmas showed 
      relatively higher antibody titer for a target antigen of death receptor 5 (DR5), 
      were selected for the source of antibody genes to construct so called an anti-DR5 
      pseudo-immune human single-chain fragment variable (scFv) library on the yeast 
      cell surface (approximately 2x10(6) diversity). Compared with a large nonimmune 
      human scFv library (approximately 1x10(9) diversity), the repertoire of the 
      pseudo-immune scFv library was significantly biased toward the target antigen, 
      which facilitated rapid enrichments of the target-specific high affinity scFvs 
      during selections by fluorescence activated cell sortings. Isolated scFvs, HW5 
      and HW6, from the pseudo-immune library showed much higher specificity and 
      affinity for the targeted antigen than those from the nonimmune library. Our 
      results suggest that a pseudo-immune antibody library is very efficient to 
      isolate target-specific high affinity antibody from a relatively small sized 
      library.
FAU - Lee, Hae-Won
AU  - Lee HW
AD  - Department of Molecular Science and Technology, Ajou University, San 5, 
      Woncheon-dong, Yeongtong-gu, Suwon 443-749, Republic of Korea.
FAU - Lee, Seung-Hyun
AU  - Lee SH
FAU - Park, Kyung-Jin
AU  - Park KJ
FAU - Kim, Jeong-Sun
AU  - Kim JS
FAU - Kwon, Myung-Hee
AU  - Kwon MH
FAU - Kim, Yong-Sung
AU  - Kim YS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060609
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Immunoglobulin Variable Region)
RN  - 0 (Peptide Library)
RN  - 0 (Receptors, TNF-Related Apoptosis-Inducing Ligand)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (TNFRSF10B protein, human)
SB  - IM
MH  - Humans
MH  - Immunoglobulin Variable Region/*genetics/immunology/isolation & 
      purification/*metabolism
MH  - *Peptide Library
MH  - Receptors, TNF-Related Apoptosis-Inducing Ligand
MH  - Receptors, Tumor Necrosis Factor/immunology
MH  - Saccharomyces cerevisiae/genetics/immunology/*metabolism
MH  - Titrimetry
EDAT- 2006/06/17 09:00
MHDA- 2006/08/22 09:00
CRDT- 2006/06/17 09:00
PHST- 2006/05/22 00:00 [received]
PHST- 2006/05/31 00:00 [accepted]
PHST- 2006/06/17 09:00 [pubmed]
PHST- 2006/08/22 09:00 [medline]
PHST- 2006/06/17 09:00 [entrez]
AID - S0006-291X(06)01278-2 [pii]
AID - 10.1016/j.bbrc.2006.05.202 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2006 Aug 4;346(3):896-903. doi: 
      10.1016/j.bbrc.2006.05.202. Epub 2006 Jun 9.