PMID- 16777070
OWN - NLM
STAT- MEDLINE
DCOM- 20060821
LR  - 20141120
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 346
IP  - 3
DP  - 2006 Aug 4
TI  - Effects of prototypical drug-metabolizing enzyme inducers on mRNA expression of 
      housekeeping genes in primary cultures of human and rat hepatocytes.
PG  - 1033-9
AB  - Quantitative real-time RT-PCR was used to investigate the effects of prototypical 
      drug-metabolizing enzyme inducers rifampicin (Rif), dexamethasone (Dex), and 
      omeprazole (Ome) on mRNA expression levels of the housekeeping genes beta-actin 
      (ACTB), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), beta-glucuronidase 
      (GUSB), hypoxanthine phosphoribosyltransferase 1 (HPRT1), peptidylprolylisomerase 
      A (PPIA), TATA box binding protein (TBP), and transferrin receptor (TFRC) in 
      primary cultures of cryopreserved human and rat hepatocytes. The mRNA levels of 
      ACTB, GAPDH, GUSB, PPIA, TBP, and TFRC relative to HPRT1 in human hepatocytes 
      were constant at all concentrations of inducers. However, the mRNA level of GAPDH 
      relative to HPRT1 in rat hepatocytes was markedly increased by Rif. The mRNA 
      levels of GAPDH, GUSB, PPIA, TBP, and TFRC relative to HPRT1 in rat hepatocytes 
      were significantly increased by Dex. ACTB and HPRT1 are suitable internal 
      controls for evaluating mRNA expression levels in primary cultures of human and 
      rat hepatocytes after Rif, Dex, or Ome exposure.
FAU - Nishimura, Masuhiro
AU  - Nishimura M
AD  - Department of Drug Metabolism, Division of Pharmacology, Drug Safety and 
      Metabolism, Otsuka Pharmaceutical Factory, Inc., Naruto, Tokushima 772-8601, 
      Japan. nisimums@otsukakj.co.jp
FAU - Koeda, Akiko
AU  - Koeda A
FAU - Suzuki, Emako
AU  - Suzuki E
FAU - Shimizu, Takefumi
AU  - Shimizu T
FAU - Kawano, Yuichi
AU  - Kawano Y
FAU - Nakayama, Mitsuo
AU  - Nakayama M
FAU - Satoh, Tetsuo
AU  - Satoh T
FAU - Narimatsu, Shizuo
AU  - Narimatsu S
FAU - Naito, Shinsaku
AU  - Naito S
LA  - eng
PT  - Journal Article
DEP - 20060609
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Pharmaceutical Preparations)
RN  - 0 (RNA, Messenger)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - KG60484QX9 (Omeprazole)
RN  - VJT6J7R4TR (Rifampin)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Cells, Cultured
MH  - Child, Preschool
MH  - Dexamethasone/*pharmacology
MH  - Female
MH  - Gene Expression Regulation/*drug effects
MH  - Genes/genetics
MH  - Hepatocytes/*drug effects/*enzymology/metabolism
MH  - Humans
MH  - Inactivation, Metabolic
MH  - Omeprazole/*pharmacology
MH  - Pharmaceutical Preparations/*metabolism
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rifampin/*pharmacology
EDAT- 2006/06/17 09:00
MHDA- 2006/08/22 09:00
CRDT- 2006/06/17 09:00
PHST- 2006/05/26 00:00 [received]
PHST- 2006/06/02 00:00 [accepted]
PHST- 2006/06/17 09:00 [pubmed]
PHST- 2006/08/22 09:00 [medline]
PHST- 2006/06/17 09:00 [entrez]
AID - S0006-291X(06)01301-5 [pii]
AID - 10.1016/j.bbrc.2006.06.012 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2006 Aug 4;346(3):1033-9. doi: 
      10.1016/j.bbrc.2006.06.012. Epub 2006 Jun 9.