PMID- 16777439
OWN - NLM
STAT- MEDLINE
DCOM- 20070105
LR  - 20060911
IS  - 1065-6995 (Print)
IS  - 1065-6995 (Linking)
VI  - 30
IP  - 9
DP  - 2006 Sep
TI  - Effects of fluid shear stress on mRNA expression of carbonic anhydrase II in 
      polarized rat osteoclasts.
PG  - 714-20
AB  - The present study was designed to determine the effects of fluid shear stress on 
      the mRNA expression of carbonic anhydrase II (CAII) in polarized rat osteoclasts. 
      Cellular morphology of the polarized osteoclasts generated by a mechanical 
      anatomical technique was examined by tartrate-resistant acid phosphatase (TRAP) 
      staining and the osteoclastic resorption of dentine slices. The polarized 
      osteoclasts were then stress-loaded by using a flow shear stress device newly 
      developed by the osteoclast research group (patent number 200420034438; China), 
      at 9 dyne/cm(2) for various time periods [0 (control group), 15, 30, 60, and 120 
      min], or at various stress levels [0 (control), 0.9, 2.9, 8.7, and 26.3 
      dyne/cm(2)] for 30 min. The mRNA expression of CAII was quantified using 
      real-time fluorescent quantitative PCR (RT-PCR) and the data were analyzed with 
      SPSS 12.0 software. The polarized osteoclasts were larger than regular monocytes 
      (about 30 microm diameter) with irregular configuration, and the majority of 
      polarized osteoclasts appeared to be spherical and had approximately 2-20 nuclei. 
      The TRAP positive polarized osteoclasts showed asymmetrical red staining in the 
      cytoplasm, and had many filaments and vacuoles. These cells formed resorptive 
      pits in dentine slices. The levels of CAII mRNA expression were shown to be 
      time-dependent, with the E+5 copy numbers being 7.88+/-0.09, 11.14+/-0.12, 
      15.83+/-0.18, 1.94+/-0.02, and 1.37+/-0.01 in cells treated at 9 dyne/cm(2) for 
      0, 15, 30, 60 and 120 min, respectively (P < 0.05). The levels of CAII mRNA 
      expression (E+5 copy numbers) in cells treated with the stress levels of 0, 0.9, 
      2.9, 8.7 and 26.3 dyne/cm(2) were 7.97+/-0.201, 11.26+/-0.688, 15.94+/-0.201, 
      31.88+/-1.496, and 45.08+/-2.639, respectively (P < 0.05). These results indicate 
      that there is a relationship between the fluid shear stress and the mRNA 
      expression of CAII in polarized rat osteoclasts.
FAU - Zhang, Qinghong
AU  - Zhang Q
AD  - Key Laboratory of Oral Biomedical Engineering of Ministry of Education, West 
      China College of Stomatology, Sichuan University, Chengdu 610041, China.
FAU - Liang, Xing
AU  - Liang X
FAU - Zhu, Baoming
AU  - Zhu B
FAU - Dong, Qiang
AU  - Dong Q
FAU - Xu, Ling
AU  - Xu L
FAU - Xia, Lu
AU  - Xia L
FAU - Hu, Jian
AU  - Hu J
FAU - Fu, Jun
AU  - Fu J
FAU - Liu, Mengtao
AU  - Liu M
LA  - eng
PT  - Journal Article
DEP - 20060512
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (RNA, Messenger)
RN  - EC 4.2.1.- (Carbonic Anhydrase II)
SB  - IM
MH  - Animals
MH  - Bone Resorption/metabolism
MH  - Carbonic Anhydrase II/genetics/*metabolism
MH  - Cells, Cultured
MH  - Male
MH  - Osteoclasts/*enzymology/metabolism/ultrastructure
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stress, Mechanical
EDAT- 2006/06/17 09:00
MHDA- 2007/01/06 09:00
CRDT- 2006/06/17 09:00
PHST- 2005/10/28 00:00 [received]
PHST- 2006/03/18 00:00 [revised]
PHST- 2006/05/03 00:00 [accepted]
PHST- 2006/06/17 09:00 [pubmed]
PHST- 2007/01/06 09:00 [medline]
PHST- 2006/06/17 09:00 [entrez]
AID - S1065-6995(06)00100-4 [pii]
AID - 10.1016/j.cellbi.2006.05.002 [doi]
PST - ppublish
SO  - Cell Biol Int. 2006 Sep;30(9):714-20. doi: 10.1016/j.cellbi.2006.05.002. Epub 
      2006 May 12.