PMID- 16778071
OWN - NLM
STAT- MEDLINE
DCOM- 20061129
LR  - 20200930
IS  - 0363-6119 (Print)
IS  - 0363-6119 (Linking)
VI  - 291
IP  - 5
DP  - 2006 Nov
TI  - Oxygen availability regulates metabolism and gene expression in trout hepatocyte 
      cultures.
PG  - R1507-15
AB  - We studied the metabolic rate, cellular energetic state, hypoxia-inducible 
      factor-1 (HIF-1) activation, and expression of enzymes involved in energy 
      metabolism using rainbow trout (Oncorhynchus mykiss) hepatocytes over the oxygen 
      range from 21 to 1 kPa. Oxygen dependence of these factors was assessed by 
      gradually reducing oxygen supply to cells from 21 kPa to 10, 5, 2, and 1 kPa. 
      Moreover, time course experiments for up to 20 h at oxygen tensions of 1 and 2 
      kPa were carried out. Reduction of oxygen from 21 kPa to 10, 5, 2, and 1 kPa 
      decreased metabolic rate of the cells by 14, 24, 37, and 46%, respectively. This 
      response was instantaneous and fully reversible upon reoxygenation. Cellular ATP 
      content and the expression of all mRNAs studied decreased when oxygen was reduced 
      from 21 to 5 and 2 kPa. The lowest ATP levels, approximately 43% of the initial 
      value, were measured at 5 kPa of oxygen, whereas the reduction in mRNA amounts 
      was most pronounced at 2 kPa. At 1 kPa oxygen tension, both ATP content and mRNA 
      amounts returned to normoxic (21 kPa) levels with a concomitant activation of 
      HIF-1, indicating reorganization of energy metabolism in adaptation of cells to 
      low oxygen supply. These results show that oxygen has a direct regulatory effect 
      on metabolism of trout hepatocyte cultures, supporting the view that oxygen has a 
      profound role in metabolic regulation in cells.
FAU - Rissanen, Eeva
AU  - Rissanen E
AD  - Centre of Excellence in Evolutionary Genetics and Physiology, Dept. of Biology, 
      Univ. of Turku, FIN-20014, Turku, Finland. eeva.rissanen@utu.fi
FAU - Tranberg, Hanna K
AU  - Tranberg HK
FAU - Nikinmaa, Mikko
AU  - Nikinmaa M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060615
PL  - United States
TA  - Am J Physiol Regul Integr Comp Physiol
JT  - American journal of physiology. Regulatory, integrative and comparative 
      physiology
JID - 100901230
RN  - 0 (Actins)
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (RNA, Messenger)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 2.7.1.2 (Glucokinase)
RN  - EC 2.7.1.40 (Pyruvate Kinase)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Actins/genetics/metabolism
MH  - Adenosine Triphosphate/genetics/metabolism
MH  - Animals
MH  - Basal Metabolism/physiology
MH  - Cell Survival/physiology
MH  - Cells, Cultured
MH  - Energy Metabolism/physiology
MH  - Gene Expression Regulation/*physiology
MH  - Glucokinase/genetics/metabolism
MH  - Hepatocytes/cytology/*metabolism
MH  - Hypoxia-Inducible Factor 1/genetics/metabolism
MH  - Metabolism/*physiology
MH  - Oncorhynchus mykiss/genetics/physiology
MH  - Oxygen/*physiology
MH  - Pyruvate Kinase/genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Time Factors
EDAT- 2006/06/17 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/06/17 09:00
PHST- 2006/06/17 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/06/17 09:00 [entrez]
AID - 00025.2006 [pii]
AID - 10.1152/ajpregu.00025.2006 [doi]
PST - ppublish
SO  - Am J Physiol Regul Integr Comp Physiol. 2006 Nov;291(5):R1507-15. doi: 
      10.1152/ajpregu.00025.2006. Epub 2006 Jun 15.