PMID- 16778285
OWN - NLM
STAT- MEDLINE
DCOM- 20060801
LR  - 20060616
IS  - 0012-3692 (Print)
IS  - 0012-3692 (Linking)
VI  - 129
IP  - 6
DP  - 2006 Jun
TI  - Multitarget fluorescence in situ hybridization elucidates equivocal lung 
      cytology.
PG  - 1629-35
AB  - The category of equivocal respiratory cytology is a common diagnostic dilemma to 
      both cytopathologists and clinicians. Chromosomal alterations are a hallmark of 
      cancer but are rare or absent in benign conditions. The goal of this study was to 
      test the ability of multitarget fluorescence in situ hybridization (FISH) for 
      dissecting equivocal respiratory cytology into reactive and malignant categories. 
      A consecutive series of 54 Papanicolaou-stained cytologic specimens of the lung 
      was analyzed. The Papanicolaou-stained atypical cell groups were photographed, 
      and the exact locations on the specimens were saved using automated stage and 
      relocation software. The specimens were hybridized with a multitarget FISH probe 
      that contains a mixture of fluorescent probes to the centromeric region of 
      chromosome 6 and to the 5p15, 8q24 (site of the MYC gene) and 7p12 (site of the 
      EGFR gene) loci. The hybridized atypical cells were selectively scored after 
      relocation. A final diagnosis was available in 45 patients, revealing lung 
      carcinoma in 55.5% (n = 25), no evidence of malignancy in 37.8% (n = 17), and 
      pulmonary metastasis of another primary carcinoma in 6.7% (n = 3). FISH results 
      were negative in all 17 patients with benign pulmonary disease and positive in 20 
      of the 25 patients (80%) with lung carcinoma (p < 0.0001). The sensitivity, 
      specificity, and positive and negative predictive values for detection of 
      malignancy were 79%, 100%, 100%, and 74%, respectively. These data suggest that 
      multitarget FISH in conjunction with automated relocation is a powerful approach 
      for the elucidation of equivocal lung cytology.
FAU - Savic, Spasenija
AU  - Savic S
AD  - Institute for Pathology, University Hospital Schonbeinstrasse 40, 4003 Basel, 
      Switzerland. ssavic@uhbs.ch
FAU - Glatz, Katharina
AU  - Glatz K
FAU - Schoenegg, Rene
AU  - Schoenegg R
FAU - Spieler, Peter
AU  - Spieler P
FAU - Feichter, Georg
AU  - Feichter G
FAU - Tamm, Michael
AU  - Tamm M
FAU - Bubendorf, Lukas
AU  - Bubendorf L
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Chest
JT  - Chest
JID - 0231335
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma/genetics/*pathology/secondary
MH  - Diagnosis, Differential
MH  - Female
MH  - Genes, erbB-1/genetics
MH  - Genes, myc/genetics
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Lung Diseases/pathology
MH  - Lung Neoplasms/genetics/*pathology/secondary
MH  - Male
MH  - Middle Aged
MH  - Predictive Value of Tests
MH  - Reproducibility of Results
EDAT- 2006/06/17 09:00
MHDA- 2006/08/02 09:00
CRDT- 2006/06/17 09:00
PHST- 2006/06/17 09:00 [pubmed]
PHST- 2006/08/02 09:00 [medline]
PHST- 2006/06/17 09:00 [entrez]
AID - S0012-3692(15)50770-8 [pii]
AID - 10.1378/chest.129.6.1629 [doi]
PST - ppublish
SO  - Chest. 2006 Jun;129(6):1629-35. doi: 10.1378/chest.129.6.1629.