PMID- 16778588
OWN - NLM
STAT- MEDLINE
DCOM- 20060727
LR  - 20191110
IS  - 1052-9551 (Print)
IS  - 1052-9551 (Linking)
VI  - 15
IP  - 2
DP  - 2006 Jun
TI  - Pitfalls in the detection of t(11;22) translocation by fluorescence in situ 
      hybridization and RT-PCR: a single-blinded study.
PG  - 83-9
AB  - The t(11;22) translocation is a diagnostic hallmark of various small round-cell 
      tumors. This study correlates the performance of fluorescence in situ 
      hybridization (FISH) and reverse transcription polymerase chain reaction (RT-PCR) 
      in the detection of this translocation analyzing paraffin-embedded tissue 
      specimens. As negative control samples, 10 cases of normal colon mucosa and 10 
      cases of colon carcinoma tissue were analyzed by FISH to determine a valid cutoff 
      value for the diagnosis of a t(11;22) translocation. The mean number of 
      false-positive nuclei differed significantly between disomic and polysomic 
      control group cases (P=0.002). Therefore, the cutoff value was determined 
      considering the pitfall polysomy. The analysis group consisted of 20 cases from 
      the University of Dusseldorf and 10 cases from the University of Bonn. These 
      cases were analyzed using PCR (Dusseldorf) and FISH (Bonn) using a single-blinded 
      approach. Twenty-two cases (73.3%) were concordant in both methods. Five cases 
      (16.7%) were discrepant, showing a positive result in FISH whereas PCR was 
      negative. Three cases (10.0%) were analyzed by FISH, and PCR failed for 
      nonoptimized tissue preparation. In conclusion, the detection of t(11;22) 
      translocation is critically dependent on a thoroughly defined cutoff value for 
      FISH and on appropriate tissue preparation for both methods. We recommend FISH as 
      a sensitive screening tool in the detection of t(11;22) followed by subsequent 
      PCR amplification of the specific chimeric transcript.
FAU - Friedrichs, Nicolaus
AU  - Friedrichs N
AD  - Institute of Pathology, University of Bonn Medical School, Sigmund-Freud-Str. 25, 
      D-53127 Bonn, Germany. nicolaus.friedrichs@ukb.uni-bonn.de
FAU - Kriegl, Lydia
AU  - Kriegl L
FAU - Poremba, Christopher
AU  - Poremba C
FAU - Schaefer, Karl-Ludwig
AU  - Schaefer KL
FAU - Gabbert, Helmut Erich
AU  - Gabbert HE
FAU - Shimomura, Akihiko
AU  - Shimomura A
FAU - Paggen, Ellen
AU  - Paggen E
FAU - Merkelbach-Bruse, Sabine
AU  - Merkelbach-Bruse S
FAU - Buettner, Reinhard
AU  - Buettner R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Multicenter Study
PL  - United States
TA  - Diagn Mol Pathol
JT  - Diagnostic molecular pathology : the American journal of surgical pathology, part 
      B
JID - 9204924
RN  - 0 (DNA Primers)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Base Sequence
MH  - Carcinoma, Small Cell/diagnosis/genetics
MH  - Case-Control Studies
MH  - Chromosomes, Human, Pair 11/*genetics
MH  - Chromosomes, Human, Pair 22/*genetics
MH  - Colonic Neoplasms/diagnosis/genetics
MH  - DNA Primers/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods/statistics & numerical data
MH  - Predictive Value of Tests
MH  - Reverse Transcriptase Polymerase Chain Reaction/*methods/statistics & numerical 
      data
MH  - Sarcoma, Small Cell/diagnosis/genetics
MH  - Single-Blind Method
MH  - *Translocation, Genetic
EDAT- 2006/06/17 09:00
MHDA- 2006/07/28 09:00
CRDT- 2006/06/17 09:00
PHST- 2006/06/17 09:00 [pubmed]
PHST- 2006/07/28 09:00 [medline]
PHST- 2006/06/17 09:00 [entrez]
AID - 00019606-200606000-00004 [pii]
AID - 10.1097/00019606-200606000-00004 [doi]
PST - ppublish
SO  - Diagn Mol Pathol. 2006 Jun;15(2):83-9. doi: 10.1097/00019606-200606000-00004.