PMID- 16780021
OWN - NLM
STAT- MEDLINE
DCOM- 20060707
LR  - 20180217
IS  - 0001-5547 (Print)
IS  - 0001-5547 (Linking)
VI  - 50
IP  - 3
DP  - 2006 May-Jun
TI  - Reliability of detecting rRNA sequences of Chlamydia trachomatis with 
      fluorescence in situ hybridization without amplification.
PG  - 277-83
AB  - OBJECTIVE: To use fluorescence in situ hybridization (FISH) using ribosomal RNA 
      (rRNA) oligonucleotide probes as the target nucleic acid for the detection of 
      Chlamydia trachomatis. STUDY DESIGN: Suitable sequences selected from the rRNA 
      sequence of C trachomatis were labeled with a fluorescent dye and used in FISH 
      for detecting chlamydial inclusion bodies and/ or elementary bodies in 
      paraformaldehyde-fixed urogenital swab samples. The sensitivity and specificity 
      of the FISH assay were compared with those of the polymerase chain reaction (PCR) 
      using plasmid primers. Positive known C trachomatis-infected McCoy cells were 
      used as positive controls. Urogenital swab specimens that were C trachomatis 
      negative on culture and PCR were used as negative controls. RESULT: Among the 128 
      samples included in the study, FISH was positive in 28 (21.8%) and PCR in 33 
      (25.7%). A significant correlation was found between the 2 detection methods. 
      Results of PCR and FISH were consistent in 115 of the 128 samples (R = 0.89). 
      Thirteen samples showed discordant results. Of these, 9 FISH negative samples 
      were PCR positive and 4 FISH positive samples were PCR negative. CONCLUSION: FISH 
      was a highly specific and fairly sensitive technique for detecting C trachomatis. 
      Signal amplification techniques and use of different fluorophores may further 
      increase the sensitivity of this technique.
FAU - Kapur, Sujala
AU  - Kapur S
AD  - Institute of Pathology, Indian Council of Medical Research, Safdarjung Hospital 
      Campus, New Delhi, India.
FAU - Abmed, Margoob
AU  - Abmed M
FAU - Singh, Vineeta
AU  - Singh V
FAU - Krupp, Guido
AU  - Krupp G
FAU - Salhan, Sudha
AU  - Salhan S
FAU - Mittal, Aruna
AU  - Mittal A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Switzerland
TA  - Acta Cytol
JT  - Acta cytologica
JID - 0370307
RN  - 0 (RNA Probes)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Bacterial Typing Techniques
MH  - Cervix Uteri/microbiology
MH  - Chlamydia Infections/diagnosis/*microbiology
MH  - Chlamydia trachomatis/genetics/*isolation & purification
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Polymerase Chain Reaction
MH  - RNA Probes
MH  - RNA, Bacterial/*genetics
MH  - RNA, Ribosomal, 16S/*genetics
MH  - Reproducibility of Results
MH  - Urethra/microbiology
EDAT- 2006/06/20 09:00
MHDA- 2006/07/11 09:00
CRDT- 2006/06/20 09:00
PHST- 2006/06/20 09:00 [pubmed]
PHST- 2006/07/11 09:00 [medline]
PHST- 2006/06/20 09:00 [entrez]
AID - 10.1159/000325954 [doi]
PST - ppublish
SO  - Acta Cytol. 2006 May-Jun;50(3):277-83. doi: 10.1159/000325954.