PMID- 16781670
OWN - NLM
STAT- MEDLINE
DCOM- 20060821
LR  - 20091119
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 346
IP  - 3
DP  - 2006 Aug 4
TI  - Platelet activation during tumor development, the potential role of BDNF-TrkB 
      autocrine loop.
PG  - 981-5
AB  - Platelets are an important place for the storage of angiogenic factors, such as 
      vascular endothelial growth factor (VEGF) and brain-derived neurotrophic factor 
      (BDNF). The present study aims to investigate the interaction between BDNF-TrkB 
      pathway and platelet activation during tumor development. In an orthotopic 
      hepatocellular carcinoma (HCC) model, increased levels of serum and plasma BDNF 
      were detected with tumor progression. Higher numbers of CD62P+ and TrkB+ 
      platelets were found in the tumor-bearing rats. In the in vitro setting, 
      tumor-conditioned-medium (TCM) and BDNF recombinant protein stimulated CD62P 
      upregulation and subsequent BDNF release in the freshly isolated platelets, 
      whereas this effect could be inhibited by TrkB blockade. TCM and BDNF culture 
      augmented the expression of heat shock protein 90 (Hsp90) in the platelets, which 
      could be reversed by TrkB blockade. In conclusion, this study suggested the 
      presence of BDNF-TrkB autocrine loop in platelets and its importance in 
      regulating platelet activation during tumor development.
FAU - Yang, Zhen Fan
AU  - Yang ZF
AD  - Centre for the Study of Liver Disease, Department of Surgery, The University of 
      Hong Kong, Pokfulam, Hong Kong, China. zfyang@hkucc.hku.hk
FAU - Ho, David W
AU  - Ho DW
FAU - Lau, Chi Keung
AU  - Lau CK
FAU - Tam, Ka Ho
AU  - Tam KH
FAU - Lam, Chi Tat
AU  - Lam CT
FAU - Poon, Ronnie T P
AU  - Poon RT
FAU - Fan, Sheung Tat
AU  - Fan ST
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060609
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - *Autocrine Communication
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cell Line, Tumor
MH  - Cell Separation
MH  - Male
MH  - Neoplasms/*metabolism/*pathology
MH  - *Platelet Activation
MH  - Protein Binding
MH  - Rats
MH  - Rats, Inbred BUF
MH  - Receptor, trkB/*metabolism
EDAT- 2006/06/20 09:00
MHDA- 2006/08/22 09:00
CRDT- 2006/06/20 09:00
PHST- 2006/05/31 00:00 [received]
PHST- 2006/06/01 00:00 [accepted]
PHST- 2006/06/20 09:00 [pubmed]
PHST- 2006/08/22 09:00 [medline]
PHST- 2006/06/20 09:00 [entrez]
AID - S0006-291X(06)01292-7 [pii]
AID - 10.1016/j.bbrc.2006.06.007 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2006 Aug 4;346(3):981-5. doi: 
      10.1016/j.bbrc.2006.06.007. Epub 2006 Jun 9.