PMID- 16781795 OWN - NLM STAT- MEDLINE DCOM- 20070423 LR - 20200930 IS - 0176-1617 (Print) IS - 0176-1617 (Linking) VI - 164 IP - 2 DP - 2007 Feb TI - Molecular cloning of a cDNA encoding a high mobility group protein in Cucumis sativus and its expression by abiotic stress treatments. PG - 205-8 AB - A cDNA encoding a high mobility group B (HMGB) protein was isolated from Cucumis sativus and characterized with respect to its sequence, expression and responses to various abiotic stress treatments. The predicted polypeptide of 146 amino acid residues contains characteristic features of HMGB family proteins including the N-terminal basic region, one HMG-box and a stretch of acidic amino acid residues at the C-terminus. In vitro nucleic acid-binding assay revealed that the HMGB protein bound to both single-stranded DNA and double-stranded DNA. DNA gel blot analysis indicated that the HMGB gene is a single copy gene in cucumber genome. RNA gel blot analysis showed that the cucumber HMGB was more abundantly expressed in the roots than in shoots and leaves. Various abiotic stresses, including cold, drought and high salinity, down regulated markedly the expression of the HMGB in cucumber. The present report identifies a novel gene encoding HMGB protein in cucumber that shows a significant response to abiotic stress treatments. FAU - Young Jang, Ji AU - Young Jang J AD - Department of Plant Biotechnology and Agricultural Plant Stress Research Center, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea. FAU - Jin Kwak, Kyung AU - Jin Kwak K FAU - Kang, Hunseung AU - Kang H LA - eng SI - GENBANK/DQ010042 PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20060615 PL - Germany TA - J Plant Physiol JT - Journal of plant physiology JID - 9882059 RN - 0 (HMGB Proteins) RN - 059QF0KO0R (Water) RN - 451W47IQ8X (Sodium Chloride) SB - IM MH - *Adaptation, Physiological MH - Amino Acid Sequence MH - Base Sequence MH - Cold Temperature MH - Cucumis sativus/genetics/*metabolism/physiology MH - Gene Expression Regulation, Plant MH - HMGB Proteins/genetics/*metabolism MH - Molecular Sequence Data MH - Sequence Analysis, DNA MH - Sodium Chloride/metabolism MH - Water/physiology EDAT- 2006/06/20 09:00 MHDA- 2007/04/24 09:00 CRDT- 2006/06/20 09:00 PHST- 2006/02/02 00:00 [received] PHST- 2006/04/13 00:00 [accepted] PHST- 2006/06/20 09:00 [pubmed] PHST- 2007/04/24 09:00 [medline] PHST- 2006/06/20 09:00 [entrez] AID - S0176-1617(06)00136-2 [pii] AID - 10.1016/j.jplph.2006.04.007 [doi] PST - ppublish SO - J Plant Physiol. 2007 Feb;164(2):205-8. doi: 10.1016/j.jplph.2006.04.007. Epub 2006 Jun 15.