PMID- 16782586
OWN - NLM
STAT- MEDLINE
DCOM- 20060823
LR  - 20191110
IS  - 1029-8428 (Print)
IS  - 1029-8428 (Linking)
VI  - 9
IP  - 4
DP  - 2006 Jun
TI  - Upregulation of brain-derived neurotrophic factor in the sensory pathway by 
      selective motor nerve injury in adult rats.
PG  - 269-83
AB  - Selective motor nerve injury by lumbar 5 ventral root transection (L5 VRT) 
      induces neuropathic pain, but the underlying mechanisms remain unknown. 
      Previously, increased expression and secretion of brain-derived neurotrophic 
      factor (BDNF) had been implicated in injury-induced neuropathic pain in the 
      sensory system. In this study, as a step to examine potential roles of BDNF in L5 
      VRT-induced neuropathic pain, we investigated BDNF gene and protein expression in 
      adult rats with L5 VRT. L5 VRT induced a dramatic upregulation of BDNF mRNA in 
      intact sensory neurons in the ipsilateral L5 dorsal root ganglia (DRG), in 
      non-neuronal cells in the ipsilateral sciatic nerve, and in motoneurons in the 
      ipsilateral spinal cord. L5 VRT also induced de novo synthesis of BDNF mRNA in 
      spinal dorsal horn neurons and in glial cells in the white matter of the 
      ipsilateral spinal cord. Consistent with the mRNA expression pattern, BDNF 
      protein was also mainly upregulated in all populations of sensory neurons in the 
      ipsilateral L5 DRG and in spinal neurons and glia. Quantitative analysis by ELISA 
      showed that the BDNF content in the DRG and sciatic nerve peaked on day 1 and 
      remained elevated 14 days after L5 VRT. These results suggest that increased BDNF 
      expression in intact primary sensory neurons and spinal cord may be an important 
      factor in the induction of neuropathic pain without axotomy of sensory neurons.
FAU - Li, Li
AU  - Li L
AD  - Department of Human Physiology and Centre for Neuroscience, Flinders University 
      of South Australia, GPO Box 2100, Adelaide 5001, Australia. l.li1@uq.edu.au
FAU - Xian, Cory J
AU  - Xian CJ
FAU - Zhong, Jin-Hua
AU  - Zhong JH
FAU - Zhou, Xin-Fu
AU  - Zhou XF
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neurotox Res
JT  - Neurotoxicity research
JID - 100929017
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis
MH  - Lumbar Vertebrae
MH  - Male
MH  - Motor Neurons/*metabolism
MH  - Neurons, Afferent/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spinal Nerve Roots/*injuries/*metabolism
MH  - Up-Regulation/*physiology
EDAT- 2006/06/20 09:00
MHDA- 2006/08/24 09:00
CRDT- 2006/06/20 09:00
PHST- 2006/06/20 09:00 [pubmed]
PHST- 2006/08/24 09:00 [medline]
PHST- 2006/06/20 09:00 [entrez]
AID - 10.1007/BF03033317 [doi]
PST - ppublish
SO  - Neurotox Res. 2006 Jun;9(4):269-83. doi: 10.1007/BF03033317.