PMID- 16783776
OWN - NLM
STAT- MEDLINE
DCOM- 20060824
LR  - 20191210
IS  - 8755-1039 (Print)
IS  - 1097-0339 (Linking)
VI  - 34
IP  - 7
DP  - 2006 Jul
TI  - An evaluation of ThinPrep UroCyte filters for the preparation of slides for 
      fluorescence in situ hybridization.
PG  - 479-84
AB  - The purpose of this study was to assess the performance of ThinPrep UroCyte 
      filters, which were designed specifically for the preparation of slides for 
      fluorescence in situ hybridization (FISH) analysis of urine specimens. One 
      hundred urine specimens were evenly split, and one portion was utilized to 
      prepare a slide with the UroCyte filter method and the other portion was used to 
      prepare a slide with a manual dropping method. All 17 of the 100 specimens 
      identified as positive by the manual method were also identified as positive with 
      the UroCyte method. No significant differences were noted in the percentage of 
      chromosomally abnormal cells (P = 0.227), cellularity (P = 0.857), signal quality 
      (P = 0.816), and DAPI counterstain quality (P = 0.369) between the two 
      methodologies. The average time taken to prepare a batch of 10 slides using the 
      UroCyte method, and that using manual method was 103 min (10.3 min/case) and 194 
      min (19.4 min/case), respectively. This study suggests that the UroCyte filter 
      method of preparing slides for FISH analysis reduces the time required to prepare 
      these slides with overall results that are similar to the currently utilized 
      manual dropping method.
CI  - Copyright 2006 Wiley-Liss, Inc.
FAU - Kipp, Benjamin R
AU  - Kipp BR
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic and Foundation, 
      Rochester, Minnesota 55905, USA.
FAU - Campion, Michael B
AU  - Campion MB
FAU - Coffman, Erin
AU  - Coffman E
FAU - Smith, Arthur
AU  - Smith A
FAU - Tomisek, John D
AU  - Tomisek JD
FAU - Browne, Gerry G
AU  - Browne GG
FAU - Panella, Jeffrey R
AU  - Panella JR
FAU - Desai, Rutuja
AU  - Desai R
FAU - Harwood, Aaron R
AU  - Harwood AR
FAU - Halling, Kevin C
AU  - Halling KC
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Diagn Cytopathol
JT  - Diagnostic cytopathology
JID - 8506895
SB  - IM
MH  - Cytodiagnosis/instrumentation/methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Reproducibility of Results
MH  - *Specimen Handling
MH  - Urinalysis/instrumentation/methods
MH  - Urinary Bladder Neoplasms/*pathology
EDAT- 2006/06/20 09:00
MHDA- 2006/08/25 09:00
CRDT- 2006/06/20 09:00
PHST- 2006/06/20 09:00 [pubmed]
PHST- 2006/08/25 09:00 [medline]
PHST- 2006/06/20 09:00 [entrez]
AID - 10.1002/dc.20488 [doi]
PST - ppublish
SO  - Diagn Cytopathol. 2006 Jul;34(7):479-84. doi: 10.1002/dc.20488.