PMID- 16786842
OWN - NLM
STAT- MEDLINE
DCOM- 20060724
LR  - 20230307
IS  - 0362-028X (Print)
IS  - 0362-028X (Linking)
VI  - 69
IP  - 6
DP  - 2006 Jun
TI  - Transfer coefficient models for escherichia coli O157:H7 on contacts between beef 
      tissue and high-density polyethylene surfaces.
PG  - 1248-55
AB  - Risk studies have identified cross-contamination during beef fabrication as a 
      knowledge gap, particularly as to how and at what levels Escherichia coli O157:H7 
      transfers among meat and cutting board (or equipment) surfaces. The objectives of 
      this study were to determine and model transfer coefficients (TCs) between E. 
      coli O157:H7 on beef tissue and high-density polyethylene (HDPE) cutting board 
      surfaces. Four different transfer scenarios were evaluated: (i) HDPE board to 
      agar, (ii) beef tissue to agar, (iii) HDPE board to beef tissue to agar, and (iv) 
      beef tissue to HDPE board to agar. Also, the following factors were studied for 
      each transfer scenario: two HDPE surface roughness levels (rough and smooth), two 
      beef tissues (fat and fascia), and two conditions of the initial beef tissue 
      inoculation with E. coli O157:H7 (wet and dry surfaces), for a total of 24 
      treatments. The TCs were calculated as a function of the plated inoculum and of 
      the cells recovered from the first contact. When the treatments were compared, 
      all of the variables evaluated interacted significantly in determining the TC. An 
      overall TC-per-treatment model did not adequately represent the reduction of the 
      cells on the original surface after each contact and the interaction of the 
      factors studied. However, an exponential model was developed that explained the 
      experimental data for all treatments and represented the recontamination of the 
      surfaces with E. coli O157:H7. The parameters for the exponential model for 
      cross-contamination with E. coli O157:H7 between beef tissue and HDPE surfaces 
      were determined, allowing for the use of the resulting model in quantitative 
      microbial risk assessment.
FAU - Flores, Rolando A
AU  - Flores RA
AD  - Crop Conversion Science and Engineering Research Unit, U.S. Department of 
      Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 
      East Mermaid Lane, Wyndmoor, Pennsylvania 19038, USA. rflores2@unl.edu
FAU - Tamplin, Mark L
AU  - Tamplin ML
FAU - Marmer, Benne S
AU  - Marmer BS
FAU - Phillips, John G
AU  - Phillips JG
FAU - Cooke, Peter H
AU  - Cooke PH
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Food Prot
JT  - Journal of food protection
JID - 7703944
RN  - 9002-88-4 (Polyethylene)
SB  - IM
MH  - Animals
MH  - Bacterial Adhesion
MH  - Cattle
MH  - Colony Count, Microbial
MH  - *Equipment Contamination
MH  - Escherichia coli O157/growth & development/*isolation & purification/physiology
MH  - Food Contamination/*analysis
MH  - Food Handling/methods
MH  - Food Microbiology
MH  - Food-Processing Industry/*standards
MH  - Meat/*microbiology
MH  - Polyethylene
MH  - Risk Assessment/*methods
EDAT- 2006/06/22 09:00
MHDA- 2006/07/25 09:00
CRDT- 2006/06/22 09:00
PHST- 2006/06/22 09:00 [pubmed]
PHST- 2006/07/25 09:00 [medline]
PHST- 2006/06/22 09:00 [entrez]
AID - S0362-028X(22)07444-0 [pii]
AID - 10.4315/0362-028x-69.6.1248 [doi]
PST - ppublish
SO  - J Food Prot. 2006 Jun;69(6):1248-55. doi: 10.4315/0362-028x-69.6.1248.