PMID- 16789416
OWN - NLM
STAT- MEDLINE
DCOM- 20060811
LR  - 20141120
IS  - 0047-1860 (Print)
IS  - 0047-1860 (Linking)
VI  - 54
IP  - 5
DP  - 2006 May
TI  - [Examination of the increased speed of detection of HER-2/neu gene amplification 
      in breast cancer by fluorescence in situ hybridization (FISH) using image 
      analysis software].
PG  - 463-5
AB  - The time required to count signals in the detection of HER-2/neu gene 
      amplification in breast cancer by fluorescence in situ hybridization (FISH) has 
      been a problem. To assess whether the amount of time necessary for counting could 
      be reduced, image analysis using computer software (Win ROOF) was tested. Five 
      photographs from each FISH sample were arranged into ten composite photographs. 
      All ten composite photographs were necessary when using the conventional method 
      of manual counting. However, using only four of the composite photographs and the 
      image analysis method, the 60 necessary nucleus numbers could be measured, and a 
      constant ratio of HER-2/neu / CEP 17 was obtained. In all 58 samples used, in the 
      presence or absence of HER-2/neu gene amplification, there was agreement in 
      counts between the conventional and image analysis methods, and a good 
      correlation of r=0.961 (p<0.001) was obtained. Using the image analysis method, 
      the necessary scoring time was reduced, particularly when the HER-2/neu gene had 
      been amplified, where it was completed in about 1/4 of the time normally 
      required. These results indicate that this image analysis method can be applied 
      when using FISH in other areas of research, and may increase the speed of 
      examination.
FAU - Kuroso, Kazuko
AU  - Kuroso K
AD  - Department of Pathology, Dokkyo Medical University Koshigaya Hospital, Koshigaya 
      343-8555.
FAU - Murakami, Shigeru
AU  - Murakami S
FAU - Jinnai, Mutsuo
AU  - Jinnai M
FAU - Ueda, Yoshihiko
AU  - Ueda Y
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PL  - Japan
TA  - Rinsho Byori
JT  - Rinsho byori. The Japanese journal of clinical pathology
JID - 2984781R
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Breast Neoplasms/*genetics
MH  - Female
MH  - *Gene Amplification
MH  - Humans
MH  - *Image Processing, Computer-Assisted
MH  - In Situ Hybridization/*methods
MH  - Receptor, ErbB-2/*genetics
MH  - Software
MH  - Time Factors
EDAT- 2006/06/23 09:00
MHDA- 2006/08/12 09:00
CRDT- 2006/06/23 09:00
PHST- 2006/06/23 09:00 [pubmed]
PHST- 2006/08/12 09:00 [medline]
PHST- 2006/06/23 09:00 [entrez]
PST - ppublish
SO  - Rinsho Byori. 2006 May;54(5):463-5.