PMID- 16790083
OWN - NLM
STAT- MEDLINE
DCOM- 20060831
LR  - 20240413
IS  - 1476-5586 (Electronic)
IS  - 1522-8002 (Print)
IS  - 1476-5586 (Linking)
VI  - 8
IP  - 5
DP  - 2006 May
TI  - HGF/SF increases tumor blood volume: a novel tool for the in vivo functional 
      molecular imaging of Met.
PG  - 344-52
AB  - Molecular functional and metabolic imaging allows visualization of 
      disease-causing processes in living organisms. Here we present a new approach for 
      the functional molecular imaging (FMI) of endogenous tyrosine kinase receptor 
      activity using Met and its ligand, hepatocyte growth factor/scatter factor 
      (HGF/SF), as a model. HGF/SF and Met play significant roles in the biology and 
      pathogenesis of a wide variety of cancers and, therefore, may serve as potential 
      targets for cancer prognosis and therapy. We have previously shown that Met 
      activation by HGF/SF increases oxygen consumption in vitro and results in 
      substantial alteration of blood oxygenation levels in vivo, as measured by blood 
      oxygenation level-dependent magnetic resonance imaging. Using contrast medium 
      (CM) ultrasound imaging, we demonstrate here that HGF/SF induces an increase in 
      tumor blood volume. This increase is evident in small vessels, including vessels 
      that were not detected before HGF/SF treatment. The specificity of the effect was 
      validated by its inhibition using anti-HGF/SF antibodies. This change in tumor 
      hemodynamics, induced by HGF/SF and measured by CM ultrasound, is further used as 
      a tool for Met FMI in tumors. This novel noninvasive molecular imaging technique 
      may be applied for the in vivo diagnosis, prognosis, and therapy of 
      Met-expressing tumors.
FAU - Tsarfaty, Galia
AU  - Tsarfaty G
AD  - Van Andel Research Institute, Grand Rapids, MI 49503, USA.
FAU - Stein, Gideon Y
AU  - Stein GY
FAU - Moshitch-Moshkovitz, Sharon
AU  - Moshitch-Moshkovitz S
FAU - Kaufman, Dafna W
AU  - Kaufman DW
FAU - Cao, Brain
AU  - Cao B
FAU - Resau, James H
AU  - Resau JH
FAU - Vande Woude, George F
AU  - Vande Woude GF
FAU - Tsarfaty, Ilan
AU  - Tsarfaty I
LA  - eng
GR  - P50 CA093990/CA/NCI NIH HHS/United States
GR  - P50CA93990/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neoplasia
JT  - Neoplasia (New York, N.Y.)
JID - 100886622
RN  - 0 (Contrast Media)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Contrast Media/pharmacology
MH  - Diagnostic Imaging/*methods
MH  - Female
MH  - Hepatocyte Growth Factor/*metabolism
MH  - Humans
MH  - Mice
MH  - Mice, Nude
MH  - Neoplasm Transplantation
MH  - Neoplasms/blood supply/*pathology
MH  - *Neovascularization, Pathologic
MH  - Oxygen/metabolism
MH  - Oxygen Consumption
MH  - Prognosis
MH  - Proto-Oncogene Proteins c-met/*metabolism
PMC - PMC1592450
EDAT- 2006/06/23 09:00
MHDA- 2006/09/01 09:00
PMCR- 2006/05/01
CRDT- 2006/06/23 09:00
PHST- 2006/06/23 09:00 [pubmed]
PHST- 2006/09/01 09:00 [medline]
PHST- 2006/06/23 09:00 [entrez]
PHST- 2006/05/01 00:00 [pmc-release]
AID - 05685 [pii]
AID - 10.1593/neo.05685 [doi]
PST - ppublish
SO  - Neoplasia. 2006 May;8(5):344-52. doi: 10.1593/neo.05685.