PMID- 16790851
OWN - NLM
STAT- MEDLINE
DCOM- 20060727
LR  - 20060622
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 333
DP  - 2006
TI  - HLA typing and its influence on organ transplantation.
PG  - 157-74
AB  - Human leukocyte antigen (HLA) molecules are expressed on almost all nucleated 
      cells, and they are the major molecules that initiate graft rejection. There are 
      three classical loci at HLA class I: HLA-A, -B, and -Cw, and five loci at class 
      II: HLA-DR, -DQ, -DP, -DM, and -DO. The system is highly polymorphic, there being 
      many alleles at each individual locus. Three methods for HLA typing are described 
      in this chapter, including serological methods and the molecular techniques of 
      sequence-specific priming (SSP) and sequence-specific oligonucleotide probing 
      (SSOP). The influence of HLA matching on solid organ and bone marrow 
      transplantation is also described. HLA matching has had the greatest clinical 
      impact in kidney and bone marrow transplantation, where efforts are made to match 
      at the HLA-A, -B, and -DR loci. In heart and lung transplantation, although 
      studies have shown it would be an advantage to match especially at the DR locus, 
      practical considerations (ischemic times, availability of donors, clinical need 
      of recipients) make this less of a consideration. Corneal grafts are not usually 
      influenced by HLA matching, unless being transplanted into a vascularized (or 
      inflamed) bed.
FAU - Sheldon, Stephen
AU  - Sheldon S
AD  - Transplantation Laboratory, Central Manchester and Manchester, Children's 
      University Hospitals NHS Trust, Manchester Royal Infirmary, Oxford Road, 
      Manchester, England.
FAU - Poulton, Kay
AU  - Poulton K
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Forecasting
MH  - HLA Antigens/*immunology
MH  - Histocompatibility Testing
MH  - Humans
MH  - *Organ Transplantation
MH  - Serology
MH  - Transplantation, Homologous
RF  - 40
EDAT- 2006/06/23 09:00
MHDA- 2006/07/28 09:00
CRDT- 2006/06/23 09:00
PHST- 2006/06/23 09:00 [pubmed]
PHST- 2006/07/28 09:00 [medline]
PHST- 2006/06/23 09:00 [entrez]
AID - 1-59745-049-9:157 [pii]
AID - 10.1385/1-59745-049-9:157 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2006;333:157-74. doi: 10.1385/1-59745-049-9:157.