PMID- 16791558
OWN - NLM
STAT- MEDLINE
DCOM- 20070504
LR  - 20160512
IS  - 1618-2642 (Print)
IS  - 1618-2642 (Linking)
VI  - 385
IP  - 5
DP  - 2006 Jul
TI  - A new time-resolved fluorometric microarray detection system using 
      core-shell-type fluorescent nanosphere and its application to allergen 
      microarray.
PG  - 797-806
AB  - We have developed a new time-resolved fluorometric (TRF) microarray detection 
      system consisting of fluorescent NH2 nanosphere, TRF microarray detector and 
      gamma-irradiated polystyrene chip. Using the TRF microarray detector, we detected 
      500 particles of the fluorescent nanosphere in one channel. Cross-talk 
      fluorescence from the adjacent channels was little observed in the TRF microarray 
      detector (<0.0004 %). The TRF microarray detection system was further applied for 
      serum allergen-specific immunoglobulin E (IgE) multi-analyses. As a labeled tag 
      antibody, an anti-human IgE Fab' fragment-conjugated fluorescent nanosphere (Fab' 
      nanosphere) was prepared as described previously. As a chip surface appropriate 
      for allergen immobilization, the polystyrene chip surface was modified by gamma 
      irradiation. The immunoassay reactivity using the gamma-irradiated polystyrene 
      chip was approximately 2.5-times improved compared with that of the non-treated 
      polystyrene chip. Non-specific adsorption of the Fab' nanosphere onto the 
      gamma-irradiated polystyrene chip surface was very low level (<0.0009 %). In only 
      20 mul of serum, six allergen-specific IgEs could be simultaneously determined in 
      one reaction well in fewer than 90 min. Good correlation curves were obtained 
      between the microarray immunoassay and the CAP RAST fluoro-enzyme immunoassay 
      (CAP/RAST FEIA) method (r > 0.961). Reproducibility (CVs) of the microarray 
      immunoassay was 8.6 % to 19.0 % (n = 5).
FAU - Matsuya, Takeshi
AU  - Matsuya T
AD  - Research & Development Division, Mitsubishi Kagaku Iatron, Inc., 1144 
      Ohwadashinden, Chiba, 276-0046, Japan. matsuya.takeshi@mm.mk-iatron.co.jp
FAU - Otake, Kazuhiko
AU  - Otake K
FAU - Tashiro, Shigeru
AU  - Tashiro S
FAU - Hoshino, Nobuhiro
AU  - Hoshino N
FAU - Katada, Motomi
AU  - Katada M
FAU - Okuyama, Tsuneo
AU  - Okuyama T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060520
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Allergens)
RN  - 0 (Polystyrenes)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*analysis/*immunology
MH  - Fluorometry
MH  - Humans
MH  - Immunoglobulin E/blood/immunology
MH  - Nanotubes/*chemistry/radiation effects
MH  - Polystyrenes/chemistry
MH  - Protein Array Analysis/*instrumentation/*methods
MH  - Reproducibility of Results
MH  - Time Factors
EDAT- 2006/06/23 09:00
MHDA- 2007/05/05 09:00
CRDT- 2006/06/23 09:00
PHST- 2005/12/06 00:00 [received]
PHST- 2006/03/27 00:00 [accepted]
PHST- 2006/03/06 00:00 [revised]
PHST- 2006/06/23 09:00 [pubmed]
PHST- 2007/05/05 09:00 [medline]
PHST- 2006/06/23 09:00 [entrez]
AID - 10.1007/s00216-006-0455-9 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2006 Jul;385(5):797-806. doi: 10.1007/s00216-006-0455-9. Epub 
      2006 May 20.