PMID- 16792867
OWN - NLM
STAT- MEDLINE
DCOM- 20070724
LR  - 20111117
IS  - 1007-3418 (Print)
IS  - 1007-3418 (Linking)
VI  - 14
IP  - 6
DP  - 2006 Jun
TI  - [Effect of tumor antigen specific CTL induced by dendritic cells on a model of 
      human hepatocellular carcinoma in nude mice (LCI-D20)].
PG  - 431-4
AB  - OBJECTIVES: To investigate the cure effect of tumor antigen specific CTL on a 
      model of human hepatocellular carcinoma in nude mice LCI-D20. METHODS: Dendritic 
      cells (DCs) were induced from peripheral blood mononuclear cells of healthy 
      people in vitro by using recombinant human granulocyte-macrophage colony 
      stimulating factor (rhGM-CSF) and interleukin-4 (rhIL-4) and were pulsed with 
      tumor antigen from hepatocellular carcinoma cell line MHCC97H. Then tumor antigen 
      specific cytotoxic T lymphocytes (CTLs) were induced. By intraperitoneal 
      injection of tumour antigen specific CTLs into the LCI-D20, the preventive and 
      therapeutic effects of these CTLs to HCC in the LCI-D20 model were assessed. 
      Cytokine-induced killer (CIK) cells and phosphate buffer solution were used as 
      controls at the same time. RESULTS: The weights of tumors in the tumor antigen 
      specific CTL group, in the CIK cell group and in the blank group were 
      (1.11+/-0.63), (1.12+/-0.36) and (2.68+/-0.53) grams respectively (t = 5.18, t = 
      6.06, P < 0.01). The amount of blood alpha fetal protein in the tumor antigen 
      specific CTL and CIK groups were (52.1+/-9.7) microg/L and (48.6+/-5.2) microg/L, 
      and was (82.2+/-7.2) microg/L in the blank group (t = 17.26, t = 22.07, P < 0.01 
      respectively). The metastasis rates in livers were 16.7%, 16.7% and 58.3% in the 
      tumor antigen specific CTL, CIK cell and blank control groups respectively (chi2= 
      4.44, P < 0.01). The survival time of the mice in the tumor antigen specific CTL 
      group was (79.0+/-5.02) days, (73.3+/-7.0) days in the CIK group, and 
      (52.3+/-5.2) days in the blank group (t = 14.56, t = 17.54, P < 0.01). 
      CONCLUSION: Tumor antigen specific CTLs may prevent metastasis in the LCI-D20 
      model and prolong the survival time.
FAU - Chen, Guo-lin
AU  - Chen GL
AD  - Institute for Viral Hepatitis, Chongqing University of Medical Sciences, 
      Chongqing 400010, China.
FAU - Hu, Huai-dong
AU  - Hu HD
FAU - Ma, Ying-ji
AU  - Ma YJ
FAU - Li, Yong-guo
AU  - Li YG
FAU - Xue, Qiong
AU  - Xue Q
FAU - Chen, Min
AU  - Chen M
FAU - Ren, Hong
AU  - Ren H
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Gan Zang Bing Za Zhi
JT  - Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of 
      hepatology
JID - 9710009
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Recombinant Proteins)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Antigens, Neoplasm/*immunology
MH  - Carcinoma, Hepatocellular/immunology/*pathology
MH  - Cell Line, Tumor
MH  - Dendritic Cells/cytology/*immunology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Interleukin-4/pharmacology
MH  - Liver Neoplasms/immunology/*pathology
MH  - Male
MH  - Mice
MH  - Mice, Nude
MH  - Neoplasm Metastasis
MH  - Neoplasm Transplantation
MH  - Recombinant Proteins
MH  - T-Lymphocytes, Cytotoxic/*immunology
EDAT- 2006/06/24 09:00
MHDA- 2007/07/25 09:00
CRDT- 2006/06/24 09:00
PHST- 2006/06/24 09:00 [pubmed]
PHST- 2007/07/25 09:00 [medline]
PHST- 2006/06/24 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Gan Zang Bing Za Zhi. 2006 Jun;14(6):431-4.