PMID- 16792917
OWN - NLM
STAT- MEDLINE
DCOM- 20090819
LR  - 20201209
IS  - 0253-2727 (Print)
IS  - 0253-2727 (Linking)
VI  - 27
IP  - 3
DP  - 2006 Mar
TI  - [Effect of anti-Helicobacter pylori ureB monoclonal antibody on platelet 
      aggregation and activation, and its mechanism study].
PG  - 166-9
AB  - OBJECTIVES: To study the effect of monoclonal antibody (McAb) against 
      helicobacter pylori (Hp) ureB, 1F11 on platelet aggregation and activation, and 
      its mechanism. METHODS: The relativity between human platelet glycoproteins (GPs) 
      and Hp ureB was identified by Western blot and FCM. Platelet aggregation was 
      measured by turbidimetry, and P-selectin and TXB2 assay by ELISA. RESULTS: 1F11 
      could bind to platelet GPIIIa, and ADP-induced platelet aggregation was inhibited 
      by 1F11 in a dose-dependent manner. However, 1F11 had no effect on plasma 
      P-selectin and TXB2 induced by ADP. The FCM results show that the positive rates 
      of platelet binding to FITC-SZ21 was decreased from 99.5% to 77.4% after addition 
      of 1F11. CONCLUSION: McAb against Hp ureB 1F11 inhibits platelet aggregation 
      through binding to platelet GPIIIa but does not block platelet activation. There 
      might be crossed-epitopes on Hp ureB and platelet GPIIIa, and Hp infection might 
      be involved in ITP immunopathology.
FAU - Bai, Yan-yan
AU  - Bai YY
AD  - Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow 
      University, Suzhou 215006, China.
FAU - Wang, Zhao-yue
AU  - Wang ZY
FAU - Bai, Xia
AU  - Bai X
FAU - Miao, Jing-cheng
AU  - Miao JC
FAU - Zhang, Wei
AU  - Zhang W
FAU - Dai, Lan
AU  - Dai L
FAU - Shen, Wen-hong
AU  - Shen WH
FAU - Ruan, Chang-geng
AU  - Ruan CG
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Integrin beta3)
RN  - 0 (P-Selectin)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
RN  - EC 3.5.1.5 (Urease)
SB  - IM
MH  - Antibodies, Bacterial/pharmacology
MH  - Antibodies, Monoclonal/*pharmacology
MH  - Bacterial Proteins/*immunology/metabolism
MH  - Helicobacter pylori/*immunology
MH  - Humans
MH  - Integrin beta3/immunology
MH  - P-Selectin/immunology
MH  - Platelet Activation/*drug effects
MH  - Platelet Aggregation/*drug effects
MH  - Urease/immunology
MH  - Urokinase-Type Plasminogen Activator/immunology
EDAT- 2006/06/24 09:00
MHDA- 2009/08/20 09:00
CRDT- 2006/06/24 09:00
PHST- 2006/06/24 09:00 [pubmed]
PHST- 2009/08/20 09:00 [medline]
PHST- 2006/06/24 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2006 Mar;27(3):166-9.