PMID- 16794255
OWN - NLM
STAT- MEDLINE
DCOM- 20061102
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 108
IP  - 8
DP  - 2006 Oct 15
TI  - DIgR2, dendritic cell-derived immunoglobulin receptor 2, is one representative of 
      a family of IgSF inhibitory receptors and mediates negative regulation of 
      dendritic cell-initiated antigen-specific T-cell responses.
PG  - 2678-86
AB  - Dendritic cells (DCs) are specialized antigen-presenting cells that play crucial 
      roles in the initiation and regulation of immune responses. Maturation and 
      activation of DCs are controlled by a balance of the inhibitory and activating 
      signals transduced through distinct surface receptors. Many inhibitory receptors 
      expressed by DCs have been identified, whereas the new members and their 
      functions need further investigation. In this study, we functionally 
      characterized DC-derived immunoglobulin receptor 2 (DIgR2) as a novel 
      representative of a family of inhibitory receptors belonging to the 
      immunoglobulin superfamily. We show that DIgR2 contains 2 immunoreceptor 
      tyrosine-based inhibitory motifs (ITIMs) within its cytoplasmic region and that 
      DIgR2 associates with Src homology-2 domain-containing protein tyrosine 
      phosphatases-1 (SHP-1). Blockade of DIgR2 on DCs by pretreatment with DIgR2-Ig 
      fusion protein or by silencing with specific small interfering RNA enhances 
      DC-initiated T-cell proliferation and antigen-specific T-cell responses both in 
      vitro and in vivo. Furthermore, immunization of mice with antigen-pulsed, 
      DIgR2-silenced DCs elicits more potent antigen-specific CD4+ and CD8+ T-cell 
      responses, thus protecting the vaccinated mice from tumor challenge more 
      effectively. Our data suggest that DIgR2 is a functionally inhibitory receptor 
      and can mediate negative signaling to regulate DC-initiated antigen-specific 
      T-cell responses.
FAU - Shi, Liyun
AU  - Shi L
AD  - Institute of Immunology and State Key Laboratory of Medical Immunology, Second 
      Military Medical University, 800 Xiangyin Road, Shanghai 200433, PR China.
FAU - Luo, Kun
AU  - Luo K
FAU - Xia, Dajing
AU  - Xia D
FAU - Chen, Taoyong
AU  - Chen T
FAU - Chen, Guoyou
AU  - Chen G
FAU - Jiang, Yingming
AU  - Jiang Y
FAU - Li, Nan
AU  - Li N
FAU - Cao, Xuetao
AU  - Cao X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060622
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Cancer Vaccines)
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Receptors, Immunologic)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cancer Vaccines/immunology/pharmacology
MH  - Cell Communication
MH  - Cell Line
MH  - Cell Proliferation
MH  - DNA, Complementary/genetics
MH  - Dendritic Cells/*immunology
MH  - In Vitro Techniques
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Molecular Sequence Data
MH  - Neoplasms, Experimental/immunology/pathology/therapy
MH  - RNA, Small Interfering/genetics
MH  - Receptors, Immunologic/antagonists & inhibitors/genetics/*metabolism
MH  - Sequence Homology, Amino Acid
MH  - T-Lymphocytes/cytology/*immunology
MH  - T-Lymphocytes, Cytotoxic/immunology
MH  - Th1 Cells/immunology
EDAT- 2006/06/24 09:00
MHDA- 2006/11/03 09:00
CRDT- 2006/06/24 09:00
PHST- 2006/06/24 09:00 [pubmed]
PHST- 2006/11/03 09:00 [medline]
PHST- 2006/06/24 09:00 [entrez]
AID - S0006-4971(20)52453-1 [pii]
AID - 10.1182/blood-2006-04-015404 [doi]
PST - ppublish
SO  - Blood. 2006 Oct 15;108(8):2678-86. doi: 10.1182/blood-2006-04-015404. Epub 2006 
      Jun 22.