PMID- 16794314
OWN - NLM
STAT- MEDLINE
DCOM- 20060831
LR  - 20151119
IS  - 0916-8451 (Print)
IS  - 0916-8451 (Linking)
VI  - 70
IP  - 6
DP  - 2006 Jun
TI  - Glycoform analysis of N-glycans linked to glycoproteins expressed in rice culture 
      cells: predominant occurrence of complex type N-glycans.
PG  - 1356-63
AB  - Although it has been found that plant endo-beta-N-acetylglucosaminidase shows 
      strong activity towards denatured glycoproteins and glycopeptides with 
      high-mannose type N-glycans and free high-mannose type N-glycans bearing the 
      chitobiosyl unit, the endogenous substrates for plant endoglycosidase have not 
      yet been identified. Recently we purified and characterized an 
      endo-beta-N-acetylglucosaminidase from rice culture cells and identified the gene 
      encoded. Furthermore, we found structural features of free N-glycans in the 
      cells, indicating that high-mannose type species (Man(9-5)GlcNAc(1)) occur at 
      concentration of several micromolar (microM). Hence, in this study we analyzed 
      glycoform of N-glycans linked to glycoproteins expressed in rice culture cells to 
      see whether endogenous glycoproteinous substrate occurs in reasonable amounts. 
      Structural analysis revealed that more than 95% of total N-glycans linked to 
      glycoproteins in the rice cells had the plant complex type structure, including 
      Lewis a epitope-harboring type, although high-mannose type structures account for 
      less than 5% of total N-glycans.
FAU - Maeda, Megumi
AU  - Maeda M
AD  - Division of Bioscience, Department of Biofunctional Chemistry, Graduate School of 
      Natural Science and Technology, Okayama University.
FAU - Kimura, Yoshinobu
AU  - Kimura Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biosci Biotechnol Biochem
JT  - Bioscience, biotechnology, and biochemistry
JID - 9205717
RN  - 0 (Glycoproteins)
RN  - 0 (Polysaccharides)
RN  - 11028-71-0 (Concanavalin A)
RN  - EC 3.2.1.- (Glycoside Hydrolases)
SB  - IM
MH  - Carbohydrate Conformation
MH  - Cell Line
MH  - Chromatography, Affinity
MH  - Chromatography, High Pressure Liquid
MH  - Concanavalin A/pharmacology
MH  - Glycoproteins/*chemistry/*metabolism
MH  - Glycoside Hydrolases/metabolism
MH  - Oryza/*cytology/*metabolism
MH  - Polysaccharides/*analysis/chemistry/*metabolism
MH  - Spectrometry, Mass, Electrospray Ionization
EDAT- 2006/06/24 09:00
MHDA- 2006/09/01 09:00
CRDT- 2006/06/24 09:00
PHST- 2006/06/24 09:00 [pubmed]
PHST- 2006/09/01 09:00 [medline]
PHST- 2006/06/24 09:00 [entrez]
AID - JST.JSTAGE/bbb/50637 [pii]
AID - 10.1271/bbb.50637 [doi]
PST - ppublish
SO  - Biosci Biotechnol Biochem. 2006 Jun;70(6):1356-63. doi: 10.1271/bbb.50637.