PMID- 16797033
OWN - NLM
STAT- MEDLINE
DCOM- 20060829
LR  - 20131121
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 79
IP  - 10
DP  - 2006 Aug 1
TI  - Effect of berberrubine on interleukin-8 and monocyte chemotactic protein-1 
      expression in human retinal pigment epithelial cell line.
PG  - 949-56
AB  - We examined the effects of berberrubine, a protoberberine alkaloid, on 
      interleukin-8 (IL-8) and monocyte chemotactic protein-1 (MCP-1) expression in a 
      human retinal pigment epithelial cell line (ARPE-19) stimulated with 
      interleukin-1beta (IL-1beta) or tumor necrosis factor alpha (TNF-alpha). ARPE-19 
      cells were cultured to confluence. Berberrubine and IL-1beta or TNF-alpha were 
      added to the medium. IL-8 and MCP-1 protein concentrations were measured using 
      enzyme-linked immunosorbent assay. IL-8 and MCP-1 mRNA were measured by real time 
      polymerase chain reaction. Nuclear factor kappaB (NF-kappaB) translocation was 
      examined by immunofluorescent staining/microscopy. Berberrubine dose-dependently 
      inhibited IL-8 and MCP-1 protein levels in the media and mRNA expression of the 
      cells stimulated with IL-1beta or TNF-alpha. Immunofluorescent 
      staining/microscopy of NF-kappaB in the nucleus of unstimulated cells was faint 
      (51+/-14 arbitrary units). Fluorescein was dense (215+/-42 or 170+/-24 arbitrary 
      units, respectively) 30 min after stimulation with IL-1beta or TNF-alpha and was 
      decreased to 62+/-18 or 47+/-16 arbitrary units, respectively, by berberrubine. 
      Berberrubine dose-dependently inhibited IL-8 and MCP-1 expression and protein 
      secretion induced by IL-1beta or TNF-alpha. Possibly, the effect on chemotactic 
      factors may be via suppression of NF-kappaB translocation.
FAU - Cui, Hu-Shan
AU  - Cui HS
AD  - Department of Ophthalmology, University of Toyama, 2630 Sugitani, Toyama 
      930-0194, Japan. ophthal@ms.toyama-mpu.ac.jp
FAU - Hayasaka, Seiji
AU  - Hayasaka S
FAU - Zhang, Xue-Yun
AU  - Zhang XY
FAU - Hayasaka, Yoriko
AU  - Hayasaka Y
FAU - Chi, Zai-Long
AU  - Chi ZL
FAU - Zheng, Lian-Shun
AU  - Zheng LS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060512
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-8)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factor RelA)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0I8Y3P32UF (Berberine)
RN  - 15401-69-1 (berberrubine)
SB  - IM
MH  - Berberine/*analogs & derivatives/pharmacology
MH  - Cell Line
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Gene Expression/drug effects
MH  - Humans
MH  - Immunohistochemistry
MH  - Interleukin-1/pharmacology
MH  - Interleukin-8/genetics/*metabolism
MH  - Molecular Structure
MH  - Pigment Epithelium of Eye/cytology/*drug effects/*metabolism
MH  - Protein Transport/drug effects
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Transcription Factor RelA/metabolism
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2006/06/27 09:00
MHDA- 2006/08/30 09:00
CRDT- 2006/06/27 09:00
PHST- 2005/12/07 00:00 [received]
PHST- 2006/04/25 00:00 [revised]
PHST- 2006/05/03 00:00 [accepted]
PHST- 2006/06/27 09:00 [pubmed]
PHST- 2006/08/30 09:00 [medline]
PHST- 2006/06/27 09:00 [entrez]
AID - S0024-3205(06)00372-9 [pii]
AID - 10.1016/j.lfs.2006.05.004 [doi]
PST - ppublish
SO  - Life Sci. 2006 Aug 1;79(10):949-56. doi: 10.1016/j.lfs.2006.05.004. Epub 2006 May 
      12.