PMID- 16797051
OWN - NLM
STAT- MEDLINE
DCOM- 20061010
LR  - 20191210
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 353
IP  - 1
DP  - 2006 Sep 15
TI  - Receptor use by pathogenic arenaviruses.
PG  - 111-20
AB  - The arenavirus family contains several important human pathogens including Lassa 
      fever virus (LASV), lymphocytic choriomeningitis virus (LCMV) and the New World 
      clade B viruses Junin (JUNV) and Machupo (MACV). Previously, alpha-dystroglycan 
      (alpha-DG) was identified as a receptor recognized by LASV and certain strains of 
      LCMV. However, other studies have suggested that alpha-DG is probably not used by 
      the clade B viruses, and the receptor(s) for these pathogens is currently 
      unknown. Using pseudotyped retroviral vectors displaying arenavirus glycoproteins 
      (GPs), we are able to explore the role played by the GP in viral entry in the 
      absence of other viral proteins. By examining the ability of the vectors to 
      transduce DG knockout murine embryonic stem (ES) cells, we have confirmed that 
      LASV has an absolute requirement for alpha-DG in these cells. However, the LCMV 
      GP can still direct substantial entry into murine ES cells in the absence of 
      alpha-DG, even when the GP from the clone 13 variant is used that has previously 
      been reported to be highly dependent on alpha-DG for entry. We also found that 
      neither LASV or LCMV pseudotyped vectors were able to transduce human or murine 
      lymphocytes, presumably due to the glycosylation state of alpha-DG in these 
      cells. In contrast, the JUNV and MACV GPs displayed broad tropism on human, 
      murine and avian cell types, including lymphocytes, and showed no requirement for 
      alpha-DG in murine ES cells. These findings highlight the importance of molecules 
      other than alpha-DG for arenavirus entry. An alternate receptor is present on 
      murine ES cells that can be used by LCMV but not by LASV, and which is not 
      available on human or murine lymphocytes, while a distinct and widely expressed 
      receptor(s) is used by the clade B viruses.
FAU - Reignier, Therese
AU  - Reignier T
AD  - Saban Research Institute of Children's Hospital Los Angeles, Los Angeles, CA 
      90027, USA.
FAU - Oldenburg, Jill
AU  - Oldenburg J
FAU - Noble, Beth
AU  - Noble B
FAU - Lamb, Erika
AU  - Lamb E
FAU - Romanowski, Victor
AU  - Romanowski V
FAU - Buchmeier, Michael J
AU  - Buchmeier MJ
FAU - Cannon, Paula M
AU  - Cannon PM
LA  - eng
GR  - AI055720/AI/NIAID NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20060621
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Virus)
SB  - IM
MH  - Animals
MH  - Arenaviridae/classification/*pathogenicity
MH  - CHO Cells
MH  - COS Cells
MH  - Cell Culture Techniques
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Cricetinae
MH  - HeLa Cells
MH  - Humans
MH  - Jurkat Cells
MH  - Mice
MH  - NIH 3T3 Cells
MH  - Receptors, Cell Surface/*metabolism
MH  - Receptors, Virus/*metabolism
MH  - Stem Cells/*cytology
MH  - Vero Cells
EDAT- 2006/06/27 09:00
MHDA- 2006/10/13 09:00
CRDT- 2006/06/27 09:00
PHST- 2006/03/02 00:00 [received]
PHST- 2006/04/11 00:00 [revised]
PHST- 2006/05/12 00:00 [accepted]
PHST- 2006/06/27 09:00 [pubmed]
PHST- 2006/10/13 09:00 [medline]
PHST- 2006/06/27 09:00 [entrez]
AID - S0042-6822(06)00337-0 [pii]
AID - 10.1016/j.virol.2006.05.018 [doi]
PST - ppublish
SO  - Virology. 2006 Sep 15;353(1):111-20. doi: 10.1016/j.virol.2006.05.018. Epub 2006 
      Jun 21.