PMID- 16797070
OWN - NLM
STAT- MEDLINE
DCOM- 20071012
LR  - 20220409
IS  - 0145-2126 (Print)
IS  - 0145-2126 (Linking)
VI  - 31
IP  - 2
DP  - 2007 Feb
TI  - Identification of PML-RARA rearrangement by RT-PCR and sequencing in an acute 
      promyelocytic leukemia without t(15;17) on G-banding and FISH.
PG  - 239-43
AB  - Acute promyelocytic leukemia (APL) is characterized by a reciprocal 
      translocation, t(15;17) (q22;q12), resulting in fusion of the genes promyelocytic 
      leukemia (PML) and retinoic acid receptor alpha (RARA). With conventional 
      cytogenetic methods, these translocations are detected in about 70-90% of 
      patients, with most of the negative results due to technical problems or cryptic 
      variants. Those masked PML-RARA fusions can be identified by molecular analyses 
      such as reverse transcriptase-polymerase chain reaction (PCR) and fluorescence in 
      situ hybridization (FISH). We have studied a patient showing morphological, 
      cytochemical, and immunophenotypic features of hypergranular APL with trisomy 8 
      as a sole anomaly. t(15;17) was not evident on FISH tests, while RT-PCR and cDNA 
      sequencing revealed the presence of PML-RARA transcripts.
FAU - Han, Jin-Yeong
AU  - Han JY
AD  - Department of Laboratory Medicine, Dong-A University College of Medicine, Busan, 
      Republic of Korea. jyhan@dau.ac.kr
FAU - Kim, Kyong-Eun
AU  - Kim KE
FAU - Kim, Kyeong-Hee
AU  - Kim KH
FAU - Park, Joo-In
AU  - Park JI
FAU - Kim, Jae-Seok
AU  - Kim JS
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060621
PL  - England
TA  - Leuk Res
JT  - Leukemia research
JID - 7706787
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (promyelocytic leukemia-retinoic acid receptor alpha fusion oncoprotein)
SB  - IM
MH  - *Chromosome Banding
MH  - Chromosomes, Human, Pair 8/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukemia, Promyelocytic, Acute/diagnosis/drug therapy/*genetics
MH  - Middle Aged
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Remission Induction
MH  - Reverse Transcriptase Polymerase Chain Reaction/*methods
MH  - Sequence Analysis, DNA/*methods
MH  - Trisomy
EDAT- 2006/06/27 09:00
MHDA- 2007/10/13 09:00
CRDT- 2006/06/27 09:00
PHST- 2006/04/13 00:00 [received]
PHST- 2006/05/09 00:00 [revised]
PHST- 2006/05/11 00:00 [accepted]
PHST- 2006/06/27 09:00 [pubmed]
PHST- 2007/10/13 09:00 [medline]
PHST- 2006/06/27 09:00 [entrez]
AID - S0145-2126(06)00165-2 [pii]
AID - 10.1016/j.leukres.2006.05.011 [doi]
PST - ppublish
SO  - Leuk Res. 2007 Feb;31(2):239-43. doi: 10.1016/j.leukres.2006.05.011. Epub 2006 
      Jun 21.