PMID- 16798115
OWN - NLM
STAT- MEDLINE
DCOM- 20061222
LR  - 20191210
IS  - 1570-0232 (Print)
IS  - 1570-0232 (Linking)
VI  - 843
IP  - 1
DP  - 2006 Oct 20
TI  - Stability studies of amphetamine and ephedrine derivatives in urine.
PG  - 84-93
AB  - Knowledge of the stability of drugs in biological specimens is a critical 
      consideration for the interpretation of analytical results. Identification of 
      proper storage conditions has been a matter of concern for most toxicology 
      laboratories (both clinical and forensic), and the stability of drugs of abuse 
      has been extensively studied. This concern should be extended to other areas of 
      analytical chemistry like antidoping control. In this work, the stability of 
      ephedrine derivatives (ephedrine, norephedrine, methylephedrine, pseudoephedrine, 
      and norpseudoephedrine), and amphetamine derivatives (amphetamine, 
      methamphetamine, 3,4-methylenedioxyamphetamine (MDA), and 
      3,4-methylenedioxymethamphetamine (MDMA)) in urine has been studied. Spiked urine 
      samples were prepared for stability testing. Urine samples were quantified by 
      GC/NPD or GC/MS. The homogeneity of each batch of sample was verified before 
      starting the stability study. The stability of analytes was evaluated in 
      sterilized and non-sterilized urine samples at different storage conditions. For 
      long-term stability testing, analyte concentration in urine stored at 4 degrees C 
      and -20 degrees C was determined at different time intervals for 24 months for 
      sterile urine samples, and for 6 months for non-sterile samples. For short-term 
      stability testing, analyte concentration was evaluated in liquid urine stored at 
      37 degrees C for 7 days. The effect of repeated freezing (at -20 degrees C) and 
      thawing (at room temperature) was also studied in sterile urine for up to three 
      cycles. No significant loss of the analytes under study was observed at any of 
      the investigated conditions. These results show the feasibility of preparing 
      reference materials containing ephedrine and amphetamine derivatives to be used 
      for quality control purposes.
FAU - Jimenez, C
AU  - Jimenez C
AD  - Unitat de Farmacologia, Institut Municipal d'Investigacio Medica, Barcelona, 
      Spain.
FAU - de la Torre, R
AU  - de la Torre R
FAU - Ventura, M
AU  - Ventura M
FAU - Segura, J
AU  - Segura J
FAU - Ventura, R
AU  - Ventura R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20060623
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - CK833KGX7E (Amphetamine)
RN  - GN83C131XS (Ephedrine)
SB  - IM
MH  - Amphetamine/*urine
MH  - Drug Stability
MH  - Ephedrine/*urine
MH  - Reference Standards
EDAT- 2006/06/27 09:00
MHDA- 2006/12/23 09:00
CRDT- 2006/06/27 09:00
PHST- 2006/03/03 00:00 [received]
PHST- 2006/05/12 00:00 [revised]
PHST- 2006/05/23 00:00 [accepted]
PHST- 2006/06/27 09:00 [pubmed]
PHST- 2006/12/23 09:00 [medline]
PHST- 2006/06/27 09:00 [entrez]
AID - S1570-0232(06)00453-3 [pii]
AID - 10.1016/j.jchromb.2006.05.036 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Oct 20;843(1):84-93. doi: 
      10.1016/j.jchromb.2006.05.036. Epub 2006 Jun 23.