PMID- 16800927
OWN - NLM
STAT- MEDLINE
DCOM- 20071016
LR  - 20171116
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 14
IP  - 3
DP  - 2006 Jun
TI  - [Effect of the NHE-1-specific inhibitor DMA on pHi, proliferation and apoptosis 
      of HL-60/ADM cells in vitro].
PG  - 488-91
AB  - The aim of this study was to evaluate the effect of dimethyl amiloride (DMA), a 
      specific inhibitor of Na(+)/H(+) exchanger-1 (NHE-1), on intracellular pH value 
      (pHi), proliferation and apoptosis of HL-60/ADM cells in vitro. After treatment 
      with DMA at different doses, pHi of HL-60 and HL-60/ADM cell lines were 
      determined by using pH-sensitive fluorescence dye BECEF-AM; the rate of growth 
      inhibition of cells was detected with MTT assay; cell cycle was detected by flow 
      cytometric DNA analysis; cell apoptosis was observed with terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL). The results 
      showed that pHi in HL-60/ADM cells was higher than that in HL-60 cells. After 
      treatment with DMA at different doses, pHi decreased, the rate of growth 
      inhibition and the rate of apoptotic cells in HL-60/ADM cells were all higher 
      than those in HL-60 cells. Meanwhile, after treatment with DMA during 100 
      micromol/L to 150 micromol/L, the increase amplitude of G(0)/G(1) phase cells and 
      the decrease amplitude of S + G(2)/M cells in HL-60/ADM cells were higher than 
      those in HL-60 cells. It is concluded that by causing intracellular 
      acidification, the NHE-1-specific inhibitor DMA inhibits proliferation of 
      HL-60/ADM cells and induces apoptosis of HL-60/ADM cells, and the degree of this 
      growth inhibition of HL-60/ADM cells is higher than that of HL-60 cells.
FAU - Chang, Cheng
AU  - Chang C
AD  - Department of Hematology, Xinqiao Hospital, The Third Military Medical 
      University, Chongqing 400037, China.
FAU - Kong, Pei-Yan
AU  - Kong PY
FAU - Chen, Xing-Hua
AU  - Chen XH
FAU - Peng, Xian-Gui
AU  - Peng XG
FAU - Liu, Lin
AU  - Liu L
FAU - Liu, Hong
AU  - Liu H
FAU - Zeng, Dong-Feng
AU  - Zeng DF
FAU - Liang, Xue
AU  - Liang X
FAU - Wang, Qing-Yu
AU  - Wang QY
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
RN  - 0 (Sodium-Hydrogen Exchangers)
RN  - 0 (growth factor-activatable Na-H exchanger NHE-1)
RN  - 3GC547293P (5-dimethylamiloride)
RN  - 7DZO8EB0Z3 (Amiloride)
RN  - 80168379AG (Doxorubicin)
SB  - IM
MH  - Amiloride/*analogs & derivatives/pharmacology
MH  - Apoptosis/*drug effects
MH  - Cell Cycle/drug effects
MH  - Cell Proliferation/*drug effects
MH  - Doxorubicin/pharmacology
MH  - Drug Resistance, Neoplasm
MH  - HL-60 Cells
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Sodium-Hydrogen Exchangers/*antagonists & inhibitors
EDAT- 2006/06/28 09:00
MHDA- 2007/10/17 09:00
CRDT- 2006/06/28 09:00
PHST- 2006/06/28 09:00 [pubmed]
PHST- 2007/10/17 09:00 [medline]
PHST- 2006/06/28 09:00 [entrez]
AID - 1009-2137(2006)03-0488-04 [pii]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2006 Jun;14(3):488-91.