PMID- 16802132
OWN - NLM
STAT- MEDLINE
DCOM- 20061024
LR  - 20220321
IS  - 0012-186X (Print)
IS  - 0012-186X (Linking)
VI  - 49
IP  - 8
DP  - 2006 Aug
TI  - Direct evidence of attempted beta cell regeneration in an 89-year-old patient 
      with recent-onset type 1 diabetes.
PG  - 1838-44
AB  - AIMS/HYPOTHESIS: We investigated whether there was evidence of attempted beta 
      cell regeneration in the pancreas obtained from a patient with recent-onset type 
      1 diabetes, and if so by what mechanism this occurred. SUBJECTS, MATERIALS AND 
      METHODS: We examined pancreas tissue from a lean 89-year-old patient (BMI 18.0 
      kg/m(2)) with recent-onset type 1 diabetes who had had a distal pancreatectomy to 
      remove a low-grade pancreatic intraepithelial neoplasia. RESULTS: In the 
      tumour-free tissue, the fractional beta cell area was 0.54+/-0.2% of pancreas 
      area (about one-third of that in non-diabetic humans). CD3-positive T lymphocytes 
      and macrophages had infiltrated the majority of the islets. Subclassification of 
      the T cell population revealed a predominance of CD8-positive cells over 
      CD4-positive cells. Beta cell apoptosis (terminal deoxynucleotidyl 
      transferase-mediated dUTP-biotin nick-end labelling [TUNEL] staining) was greatly 
      increased, consistent with ongoing immune-mediated beta cell destruction. There 
      was also a marked increase (more than approximately 100-fold) in the frequency of 
      beta cell replication (0.69+/-0.15% Ki67-positive beta cells) in all blocks 
      examined. CONCLUSIONS/INTERPRETATION: The present report provides direct evidence 
      of attempted beta cell regeneration through the mechanism of beta cell 
      replication in a case of newly diagnosed type 1 diabetes, and affirms that beta 
      cell apoptosis is an important mechanism for beta cell loss in type 1 diabetes.
FAU - Meier, J J
AU  - Meier JJ
AD  - Larry Hillblom Islet Research Center, UCLA David Geffen School of Medicine, 
      University of California-Los Angeles, 900 Veteran Avenue, Los Angeles, CA 
      90095-7073, USA.
FAU - Lin, J C
AU  - Lin JC
FAU - Butler, A E
AU  - Butler AE
FAU - Galasso, R
AU  - Galasso R
FAU - Martinez, D S
AU  - Martinez DS
FAU - Butler, P C
AU  - Butler PC
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060627
PL  - Germany
TA  - Diabetologia
JT  - Diabetologia
JID - 0006777
SB  - IM
CIN - Diabetologia. 2006 Nov;49(11):2801-2; author reply 2803-4. PMID: 16951935
MH  - Age of Onset
MH  - Aged, 80 and over
MH  - Diabetes Mellitus, Type 1/*physiopathology
MH  - Humans
MH  - Islets of Langerhans/metabolism/pathology/*physiopathology
MH  - Male
MH  - Pancreatectomy
MH  - Pancreatic Neoplasms/pathology/surgery
MH  - Regeneration
EDAT- 2006/06/28 09:00
MHDA- 2006/10/25 09:00
CRDT- 2006/06/28 09:00
PHST- 2006/03/20 00:00 [received]
PHST- 2006/04/08 00:00 [accepted]
PHST- 2006/06/28 09:00 [pubmed]
PHST- 2006/10/25 09:00 [medline]
PHST- 2006/06/28 09:00 [entrez]
AID - 10.1007/s00125-006-0308-2 [doi]
PST - ppublish
SO  - Diabetologia. 2006 Aug;49(8):1838-44. doi: 10.1007/s00125-006-0308-2. Epub 2006 
      Jun 27.