PMID- 1680549
OWN - NLM
STAT- MEDLINE
DCOM- 19911029
LR  - 20071114
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 51
IP  - 19
DP  - 1991 Oct 1
TI  - Molecular genetic alterations in superficial and locally advanced human bladder 
      cancer.
PG  - 5405-9
AB  - We attempted to define the role of tumor suppressor genes in the pathogenesis of 
      human bladder cancer through a combined molecular genetic and immunohistochemical 
      approach. Thirty-four bladder tumors (1 Pis, 6 Pa, 5 P1, 3 P3a, 18 P3b, 1 P4; 8 
      low grade and 26 high grade tumors) have been analyzed. Restriction fragment 
      length polymorphism analysis directed at 5 suspected or established tumor 
      suppressor gene regions (3p21-25, 11p15, 13q14, 17p11-13, and 18q21) was combined 
      with immunohistochemical using Rb-PMG3-245 monoclonal antibody directed at the 
      retinoblastoma (Rb) gene product. Tumor grade correlated with deletions of 3p (P 
      = 0.004) and 17p (P = 0.063). Tumor stage correlated with deletions of 3p (P = 
      0.010), 17p (P = 0.015) and altered Rb expression (P = 0.054). Vascular invasion 
      correlated only with deletions of 17p (P = 0.038). No marker correlated with 
      positive lymph nodes. Our results suggest that altered Rb expression occurs in 
      all grades and stages of bladder cancer but is more commonly associated with 
      invasive tumors. Genetic alterations of 3p, 11p, 17p, and 18q are rare events in 
      low grade, superficial tumors, whereas they are more common in high grade and 
      invasive bladder cancer. The role of these genetic alterations in the prognosis 
      of bladder cancer will require additional follow-up and further studies.
FAU - Presti, J C Jr
AU  - Presti JC Jr
AD  - Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York 
      10021.
FAU - Reuter, V E
AU  - Reuter VE
FAU - Galan, T
AU  - Galan T
FAU - Fair, W R
AU  - Fair WR
FAU - Cordon-Cardo, C
AU  - Cordon-Cardo C
LA  - eng
GR  - CA47538/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (Retinoblastoma Protein)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Blotting, Southern
MH  - Chromosome Deletion
MH  - Chromosomes, Human, Pair 11
MH  - Chromosomes, Human, Pair 13
MH  - Chromosomes, Human, Pair 17
MH  - Chromosomes, Human, Pair 18
MH  - Chromosomes, Human, Pair 3
MH  - DNA/analysis
MH  - Genes, Tumor Suppressor
MH  - Humans
MH  - Immunohistochemistry
MH  - Neoplasm Invasiveness
MH  - Neoplasm Staging
MH  - Polymorphism, Restriction Fragment Length
MH  - Retinoblastoma Protein/biosynthesis
MH  - Urinary Bladder Neoplasms/*genetics/metabolism
EDAT- 1991/10/11 19:15
MHDA- 2001/03/28 10:01
CRDT- 1991/10/11 19:15
PHST- 1991/10/11 19:15 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1991/10/11 19:15 [entrez]
PST - ppublish
SO  - Cancer Res. 1991 Oct 1;51(19):5405-9.