PMID- 16806267
OWN - NLM
STAT- MEDLINE
DCOM- 20060926
LR  - 20161124
IS  - 0022-2836 (Print)
IS  - 0022-2836 (Linking)
VI  - 360
IP  - 5
DP  - 2006 Jul 28
TI  - Crystal structure of the catalytic domain of human MAP kinase phosphatase 5: 
      structural insight into constitutively active phosphatase.
PG  - 946-55
AB  - MAP kinase phosphatase 5 (MKP5) is a member of the mitogen-activated protein 
      kinase phosphatase (MKP) family and selectively dephosphorylates JNK and p38. We 
      have determined the crystal structure of the catalytic domain of human MKP5 
      (MKP5-C) to 1.6 A. In previously reported MKP-C structures, the residues that 
      constitute the active site are seriously deviated from the active conformation of 
      protein tyrosine phosphatases (PTPs), which are accompanied by low catalytic 
      activity. High activities of MKPs are achieved by binding their cognate 
      substrates, representing substrate-induced activation. However, the MKP5-C 
      structure adopts an active conformation of PTP even in the absence of its 
      substrate binding, which is consistent with the previous results that MKP5 solely 
      possesses the intrinsic activity. Further, we identify a sequence motif common to 
      the members of MKPs having low catalytic activity by comparing structures and 
      sequences of other MKPs. Our structural information provides an explanation of 
      constitutive activity of MKP5 as well as the structural insight into 
      substrate-induced activation occurred in other MKPs.
FAU - Jeong, Dae Gwin
AU  - Jeong DG
AD  - Systemic Proteomics Research Center, Korea Research Institute of Bioscience and 
      Biotechnology, 52 Eoeun-Dong, Yuseong-Gu, Daejeon, 305-333, Republic of Korea.
FAU - Yoon, Tae-Sung
AU  - Yoon TS
FAU - Kim, Jae Hoon
AU  - Kim JH
FAU - Shim, Mi Young
AU  - Shim MY
FAU - Jung, Suk-Kyeong
AU  - Jung SK
FAU - Son, Jeong Hee
AU  - Son JH
FAU - Ryu, Seong Eon
AU  - Ryu SE
FAU - Kim, Seung Jun
AU  - Kim SJ
LA  - eng
SI  - PDB/1ZZW
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060609
PL  - Netherlands
TA  - J Mol Biol
JT  - Journal of molecular biology
JID - 2985088R
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - EC 3.1.3.16 (DUSP10 protein, human)
RN  - EC 3.1.3.16 (Mitogen-Activated Protein Kinase Phosphatases)
RN  - EC 3.1.3.48 (Dual-Specificity Phosphatases)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Catalysis
MH  - Catalytic Domain
MH  - Crystallography, X-Ray
MH  - Dual-Specificity Phosphatases
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - Mitogen-Activated Protein Kinase Phosphatases
MH  - *Models, Molecular
MH  - Molecular Sequence Data
MH  - Protein Conformation
MH  - Protein Tyrosine Phosphatases/*chemistry
MH  - Sequence Homology, Amino Acid
MH  - Substrate Specificity
EDAT- 2006/06/30 09:00
MHDA- 2006/09/27 09:00
CRDT- 2006/06/30 09:00
PHST- 2006/01/17 00:00 [received]
PHST- 2006/04/26 00:00 [revised]
PHST- 2006/05/23 00:00 [accepted]
PHST- 2006/06/30 09:00 [pubmed]
PHST- 2006/09/27 09:00 [medline]
PHST- 2006/06/30 09:00 [entrez]
AID - S0022-2836(06)00673-5 [pii]
AID - 10.1016/j.jmb.2006.05.059 [doi]
PST - ppublish
SO  - J Mol Biol. 2006 Jul 28;360(5):946-55. doi: 10.1016/j.jmb.2006.05.059. Epub 2006 
      Jun 9.