PMID- 16806384
OWN - NLM
STAT- MEDLINE
DCOM- 20061010
LR  - 20141120
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 353
IP  - 1
DP  - 2006 Sep 15
TI  - Adeno-associated virus (AAV) capsid genes isolated from rat and mouse liver 
      genomic DNA define two new AAV species distantly related to AAV-5.
PG  - 68-82
AB  - Using polymerase chain reactions and genome walking strategies, adeno-associated 
      virus (AAV)-like capsid genes were isolated from rat and mouse liver genomic DNA, 
      where they are present at <5 copies per cell. These genes define two new species 
      of AAVs since their amino acid sequences are <60% identical to each other or to 
      any other AAV capsid. They are most similar to the AAV-5 and goat AAV capsids. A 
      recombinant vector with the mouse AAV capsid and a lacZ transgene (rAAV-mo.1 
      lacZ) was able to transduce rodent cell lines in vitro. However, it was not able 
      to transduce eight human cell lines or primary human fibroblasts in vitro. It did 
      not bind heparin and its ability to transduce cells in vitro was not inhibited by 
      heparin, mucin, or sialic acid suggesting it uses a novel entry receptor. 
      rAAV-mo.1 lacZ was 29 times more resistant to in vitro neutralization by pooled, 
      purified human IgG than AAV-2. In vivo, rAAV-mo.1 lacZ efficiently transduced 
      murine ocular cells after a subretinal injection. Intramuscular injection of a 
      rAAV-mo.1 human factor IX (hFIX) vector into mice resulted in no detectable hFIX 
      in plasma, but intravenous injection resulted in high plasma levels of hFIX, 
      equivalent to that obtained from a rAAV-8 hFIX vector. Biodistribution analysis 
      showed that rAAV-mo.1 primarily transduced liver after an intravenous injection. 
      These AAV capsids may be useful for gene transfer in rodents.
FAU - Lochrie, Michael A
AU  - Lochrie MA
AD  - Avigen, Inc., 1301 Harbor Bay Parkway, Alameda, CA 94502-6541, USA. 
      mlochrie@synergenics.net
FAU - Tatsuno, Gwen P
AU  - Tatsuno GP
FAU - Arbetman, Alejandra E
AU  - Arbetman AE
FAU - Jones, Kris
AU  - Jones K
FAU - Pater, Cheryl
AU  - Pater C
FAU - Smith, Peter H
AU  - Smith PH
FAU - McDonnell, Jennifer W
AU  - McDonnell JW
FAU - Zhou, Shang-Zhen
AU  - Zhou SZ
FAU - Kachi, Shu
AU  - Kachi S
FAU - Kachi, Michiko
AU  - Kachi M
FAU - Campochiaro, Peter A
AU  - Campochiaro PA
FAU - Pierce, Glenn F
AU  - Pierce GF
FAU - Colosi, Peter
AU  - Colosi P
LA  - eng
GR  - EY12609/EY/NEI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20060630
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Capsid/chemistry/*physiology
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Conserved Sequence
MH  - DNA, Viral/genetics/*isolation & purification
MH  - Dependovirus/chemistry/*genetics
MH  - Gene Dosage
MH  - HeLa Cells
MH  - Humans
MH  - In Vitro Techniques
MH  - Liver/*virology
MH  - Mice
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - NIH 3T3 Cells
MH  - Neutralization Tests
MH  - Protein Structure, Tertiary
MH  - Rats
MH  - Sequence Homology, Amino Acid
MH  - Species Specificity
MH  - Tissue Distribution
MH  - Transduction, Genetic
EDAT- 2006/06/30 09:00
MHDA- 2006/10/13 09:00
CRDT- 2006/06/30 09:00
PHST- 2006/02/26 00:00 [received]
PHST- 2006/04/24 00:00 [revised]
PHST- 2006/05/18 00:00 [accepted]
PHST- 2006/06/30 09:00 [pubmed]
PHST- 2006/10/13 09:00 [medline]
PHST- 2006/06/30 09:00 [entrez]
AID - S0042-6822(06)00354-0 [pii]
AID - 10.1016/j.virol.2006.05.023 [doi]
PST - ppublish
SO  - Virology. 2006 Sep 15;353(1):68-82. doi: 10.1016/j.virol.2006.05.023. Epub 2006 
      Jun 30.