PMID- 16806476
OWN - NLM
STAT- MEDLINE
DCOM- 20070305
LR  - 20190816
IS  - 0161-5890 (Print)
IS  - 0161-5890 (Linking)
VI  - 44
IP  - 6
DP  - 2007 Feb
TI  - Saxatilin inhibits TNF-alpha-induced proliferation by suppressing AP-1-dependent 
      IL-8 expression in the ovarian cancer cell line MDAH 2774.
PG  - 1409-16
AB  - Tumor necrosis factor-alpha (TNF-alpha)-induced proliferation of cancer cell line 
      MDAH 2774 was significantly suppressed by treating the cells with saxatilin, a 
      snake venom disintegrin. The suppressed proliferation was found to be associated 
      with the level of interleukin-8 (IL-8) expression in the cells. TNF-alpha-induced 
      IL-8 promoter activation that is inhibited by saxatilin treatment was dependent 
      on activating protein-1 (AP-1) instead of nuclear factor-kappa B (NF-kappaB). 
      Coexpression of dominant negative p38 (DN-p38) suggested that p38 is involved in 
      the IL-8 promoter activity which is regulated by saxatilin or TNF-alpha. 
      Experimental evidence clearly indicated that saxatilin inhibits TNF-alpha-induced 
      proliferation of the ovarian cancer cells by suppressing IL-8 expression in 
      AP-1-dependent manner.
FAU - Kim, Dong Seok
AU  - Kim DS
AD  - Department of Biochemistry, College of Science, Yonsei University, 134 
      Sinchon-dong, Seodaemun-gu, Seoul 120-749, Republic of Korea.
FAU - Jang, Yoon-Jung
AU  - Jang YJ
FAU - Jeon, Ok-Hee
AU  - Jeon OH
FAU - Kim, Doo-Sik
AU  - Kim DS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060627
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (Antineoplastic Agents)
RN  - 0 (CXCL8 protein, human)
RN  - 0 (Disintegrins)
RN  - 0 (Growth Inhibitors)
RN  - 0 (Interleukin-8)
RN  - 0 (Transcription Factor AP-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (saxatilin)
SB  - IM
MH  - Antineoplastic Agents/*therapeutic use
MH  - Cell Line, Tumor
MH  - *Cell Proliferation
MH  - Disintegrins/*therapeutic use
MH  - Female
MH  - Growth Inhibitors/*therapeutic use
MH  - Humans
MH  - Interleukin-8/*antagonists & inhibitors/biosynthesis/genetics
MH  - Ovarian Neoplasms/*drug therapy/immunology
MH  - Transcription Factor AP-1/*antagonists & inhibitors/physiology
MH  - Tumor Necrosis Factor-alpha/*antagonists & inhibitors/physiology
EDAT- 2006/06/30 09:00
MHDA- 2007/03/06 09:00
CRDT- 2006/06/30 09:00
PHST- 2006/02/27 00:00 [received]
PHST- 2006/04/29 00:00 [revised]
PHST- 2006/05/01 00:00 [accepted]
PHST- 2006/06/30 09:00 [pubmed]
PHST- 2007/03/06 09:00 [medline]
PHST- 2006/06/30 09:00 [entrez]
AID - S0161-5890(06)00177-5 [pii]
AID - 10.1016/j.molimm.2006.05.001 [doi]
PST - ppublish
SO  - Mol Immunol. 2007 Feb;44(6):1409-16. doi: 10.1016/j.molimm.2006.05.001. Epub 2006 
      Jun 27.