PMID- 16806502
OWN - NLM
STAT- MEDLINE
DCOM- 20061031
LR  - 20061115
IS  - 0166-0934 (Print)
IS  - 0166-0934 (Linking)
VI  - 136
IP  - 1-2
DP  - 2006 Sep
TI  - Epstein-Barr virus integrates frequently into chromosome 4q, 2q, 1q and 7q of 
      Burkitt's lymphoma cell line (Raji).
PG  - 193-9
AB  - Epstein-Barr virus (EBV) integration into a Burkitt's lymphoma (BL) cell line 
      (Raji) was investigated, using polymerase chain reaction (PCR), Southern 
      hybridization, genomic library screening and fluorescence in situ hybridization 
      (FISH). BaMHIW fragments of the EBV genome and DNA sequences of the viral latent 
      membrane protein (LMP)1 and LMP2 genes were detected in Raji cells. 
      BaMHI-digested high-molecular weight DNA from Raji cells generated 4 and 10 kb, 
      23 kb fragments that hybridized to Probe-1 (EBV genome 13232-16189) and Probe-2 
      (EBV genome 5-3271). Genomic library for Raji cells was constructed. Plaques (1 x 
      10(5)) were screened with Probe-2, and four positive clones were obtained. 
      Chromosomal integration of EBV DNA was detected in the Raji cell. The viral 
      integration sites included 1p, 1q, 2q, 3p, 3q, 4q, 5q, 6q, 7p, 7q, 9q, 11p, 14q 
      and 15q. Despite this multiplicity of integration sites, integration showed high 
      frequency only at the sites 4q, 2q, 1q and 7q; 64% of the total signals were 
      found in these four chromosomal bands. No viral integration occurred in 
      chromosomes 16-22 or the sex chromosomes (X, Y). This study is the first 
      comprehensive FISH analysis of EBV integration into the chromosomes of the Raji 
      cell line. The findings support the notion that EBV integrates into the Raji cell 
      genome non-randomly.
FAU - Gao, Jianming
AU  - Gao J
AD  - Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, 
      Changsha, Hunan 410078, China.
FAU - Luo, Xiaomin
AU  - Luo X
FAU - Tang, Ke
AU  - Tang K
FAU - Li, Xiaoling
AU  - Li X
FAU - Li, Guiyuan
AU  - Li G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060623
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (DNA, Viral)
RN  - 0 (EBV-associated membrane antigen, Epstein-Barr virus)
RN  - 0 (Viral Matrix Proteins)
SB  - IM
MH  - Adult
MH  - Blotting, Southern
MH  - Burkitt Lymphoma/*virology
MH  - Cell Line
MH  - Chromosomes, Human, Pair 1
MH  - Chromosomes, Human, Pair 2
MH  - Chromosomes, Human, Pair 4
MH  - DNA, Viral/genetics
MH  - Female
MH  - Genomic Library
MH  - Herpesvirus 4, Human/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Proviruses/genetics
MH  - Viral Matrix Proteins/genetics
MH  - *Virus Integration
EDAT- 2006/06/30 09:00
MHDA- 2006/11/01 09:00
CRDT- 2006/06/30 09:00
PHST- 2006/03/31 00:00 [received]
PHST- 2006/05/08 00:00 [revised]
PHST- 2006/05/11 00:00 [accepted]
PHST- 2006/06/30 09:00 [pubmed]
PHST- 2006/11/01 09:00 [medline]
PHST- 2006/06/30 09:00 [entrez]
AID - S0166-0934(06)00175-3 [pii]
AID - 10.1016/j.jviromet.2006.05.013 [doi]
PST - ppublish
SO  - J Virol Methods. 2006 Sep;136(1-2):193-9. doi: 10.1016/j.jviromet.2006.05.013. 
      Epub 2006 Jun 23.