PMID- 16807145
OWN - NLM
STAT- MEDLINE
DCOM- 20061025
LR  - 20220408
IS  - 1570-0232 (Print)
IS  - 1570-0232 (Linking)
VI  - 842
IP  - 1
DP  - 2006 Sep 14
TI  - Simultaneous determination of plasmatic phytosterols and cholesterol precursors 
      using gas chromatography-mass spectrometry (GC-MS) with selective ion monitoring 
      (SIM).
PG  - 43-7
AB  - Phytosterols (beta-sitosterol, cholestanol and campesterol) and cholesterol 
      precursors (desmosterol and lathosterol), have been suggested as important 
      biochemical markers of intestinal cholesterol absorption and liver biosynthesis, 
      respectively, and as useful clinical parameters in the study of 
      hypercholesterolemia, beta-sitosterolemia, atherosclerosis and cardiovascular 
      disease, including pharmacological response to hypolipidemic agents. We developed 
      an optimised analytical method for the simultaneous analysis of cholestanol, 
      desmosterol, lathosterol, campesterol and beta-sitosterol in plasma using 
      capillary gas chromatography coupled to mass spectrometry (GC-MS) with multiple 
      selected ion monitoring (SIM). This method is based on the alkaline hydrolysis of 
      sterol esters, extraction of free sterols and derivatization. The recovery of all 
      sterols was in the range 76-101%. Within-day relative standard deviations 
      (R.S.Ds.) and the between-day R.S.Ds. of cholestanol, desmosterol, lathosterol, 
      campesterol and beta-sitosterol were less than 8%, and their plasma levels in 161 
      normal subjects were (mean+/-S.D.) 4.73+/-2.57, 2.37+/-1.04, 6.23+/-3.14, 
      3.67+/-1.95 and 5.92+/-3.62 micromol/l, respectively.
FAU - Ahmida, H S Mohamed
AU  - Ahmida HS
AD  - Department of Internal Medicine, University of Rome, and Department of Clinical 
      Biochemistry, Tor Vergata Hospital, Tor Vergata, Via Montpellier 1, 00133 Rome, 
      Italy.
FAU - Bertucci, Pierfrancesco
AU  - Bertucci P
FAU - Franzo, Letizia
AU  - Franzo L
FAU - Massoud, Renato
AU  - Massoud R
FAU - Cortese, Claudio
AU  - Cortese C
FAU - Lala, Alberto
AU  - Lala A
FAU - Federici, Giorgio
AU  - Federici G
LA  - eng
PT  - Journal Article
DEP - 20060627
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Phytosterols)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Cholesterol/*blood/chemistry
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Humans
MH  - Phytosterols/*blood
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
EDAT- 2006/06/30 09:00
MHDA- 2006/10/26 09:00
CRDT- 2006/06/30 09:00
PHST- 2005/08/04 00:00 [received]
PHST- 2006/04/12 00:00 [revised]
PHST- 2006/05/04 00:00 [accepted]
PHST- 2006/06/30 09:00 [pubmed]
PHST- 2006/10/26 09:00 [medline]
PHST- 2006/06/30 09:00 [entrez]
AID - S1570-0232(06)00406-5 [pii]
AID - 10.1016/j.jchromb.2006.05.024 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Sep 14;842(1):43-7. doi: 
      10.1016/j.jchromb.2006.05.024. Epub 2006 Jun 27.