PMID- 16807303
OWN - NLM
STAT- MEDLINE
DCOM- 20070215
LR  - 20220408
IS  - 0363-6143 (Print)
IS  - 0363-6143 (Linking)
VI  - 291
IP  - 6
DP  - 2006 Dec
TI  - ICAM-1 expression in adipose tissue: effects of diet-induced obesity in mice.
PG  - C1232-9
AB  - Obesity has been linked to cardiovascular disease, hypertension, diabetes and the 
      metabolic syndrome, with elevated markers of systemic inflammation. Intercellular 
      adhesion molecule-1 (ICAM-1) is a transmembrane adhesion molecule involved in 
      leukocyte migration to sites of inflammation. In human obesity, elevated 
      expression of the soluble form of ICAM-1 (sICAM-1) is positively correlated with 
      abdominal fat deposition. Increases in adiposity have also been correlated with 
      macrophage infiltration into adipose tissue. Here we investigate adipose tissue 
      production and transcriptional regulation of ICAM-1 in a mouse model of dietary 
      obesity. After feeding mice a high-fat diet, ICAM-1 expression in serum and 
      adipose tissue was analyzed by ELISA, Northern blotting, real-time quantitative 
      PCR, and flow cytometry. After 6 mo on the high-fat diet, sICAM-1 levels 
      significantly correlated with body weight and abdominal fat mass. ICAM-1 mRNA was 
      expressed in adipose tissue of mice, with significantly higher levels in males 
      than females. After only 3 wk, there were adipose tissue-specific increases in 
      mRNAs for ICAM-1, IL-6, and monocyte chemoattractant protein-1 (MCP-1) in male 
      mice. Analysis of the stromal-vascular fraction of male adipose tissue revealed 
      CD11b-negative cells with increased surface ICAM-1 and CD34. We also found two 
      populations of F4/80+, CD11b+, ICAM-1+ cells, one of which also expressed CD14 
      and CD11c and was increased in response to a high-fat diet. These results 
      indicate that within 3 wk on a high-fat diet, male mice exhibited significant 
      increases in pro-inflammatory factors and immune cell infiltration in adipose 
      tissue that may represent links between obesity and its associated inflammatory 
      complications.
FAU - Brake, Danett K
AU  - Brake DK
AD  - Section of Leukocyte Biology, Children's Nutrition Research Center, Department of 
      Pediatrics, Baylor College of Medicine, Houston, TX 77030-2600, USA.
FAU - Smith, E O'Brian
AU  - Smith EO
FAU - Mersmann, Harry
AU  - Mersmann H
FAU - Smith, C Wayne
AU  - Smith CW
FAU - Robker, Rebecca L
AU  - Robker RL
LA  - eng
GR  - DK07664/DK/NIDDK NIH HHS/United States
GR  - DK60381/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20060628
PL  - United States
TA  - Am J Physiol Cell Physiol
JT  - American journal of physiology. Cell physiology
JID - 100901225
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Cytokines)
RN  - 0 (Dietary Fats)
RN  - 0 (Protein Isoforms)
RN  - 0 (monocyte-macrophage differentiation antigen)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Adipose Tissue/cytology/*metabolism
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Antigens, Differentiation/metabolism
MH  - Body Weight
MH  - Cytokines/metabolism
MH  - *Diet
MH  - Dietary Fats
MH  - Female
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/genetics/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Obesity/immunology/*metabolism
MH  - Protein Isoforms/genetics/metabolism
MH  - Transcription, Genetic
EDAT- 2006/06/30 09:00
MHDA- 2007/02/16 09:00
CRDT- 2006/06/30 09:00
PHST- 2006/06/30 09:00 [pubmed]
PHST- 2007/02/16 09:00 [medline]
PHST- 2006/06/30 09:00 [entrez]
AID - 00008.2006 [pii]
AID - 10.1152/ajpcell.00008.2006 [doi]
PST - ppublish
SO  - Am J Physiol Cell Physiol. 2006 Dec;291(6):C1232-9. doi: 
      10.1152/ajpcell.00008.2006. Epub 2006 Jun 28.