PMID- 16808614
OWN - NLM
STAT- MEDLINE
DCOM- 20060816
LR  - 20220330
IS  - 0033-7587 (Print)
IS  - 0033-7587 (Linking)
VI  - 166
IP  - 1 Pt 2
DP  - 2006 Jul
TI  - Retrospective assessment of radiation exposure using biological dosimetry: 
      chromosome painting, electron paramagnetic resonance and the glycophorin a 
      mutation assay.
PG  - 287-302
AB  - Biological monitoring of dose can contribute important, independent estimates of 
      cumulative radiation exposure in epidemiological studies, especially in studies 
      in which the physical dosimetry is lacking. Three biodosimeters that have been 
      used in epidemiological studies to estimate past radiation exposure from external 
      sources will be highlighted: chromosome painting or FISH (fluorescence in situ 
      hybridization), the glycophorin A somatic mutation assay (GPA), and electron 
      paramagnetic resonance (EPR) with teeth. All three biodosimeters have been 
      applied to A-bomb survivors, Chernobyl clean-up workers, and radiation workers. 
      Each biodosimeter has unique advantages and limitations depending upon the level 
      and type of radiation exposure. Chromosome painting has been the most widely 
      applied biodosimeter in epidemiological studies of past radiation exposure, and 
      results of these studies provide evidence that dose-related translocations 
      persist for decades. EPR tooth dosimetry has been used to validate dose models of 
      acute and chronic radiation exposure, although the present requirement of 
      extracted teeth has been a disadvantage. GPA has been correlated with physically 
      based radiation dose after high-dose, acute exposures but not after low-dose, 
      chronic exposures. Interindividual variability appears to be a limitation for 
      both chromosome painting and GPA. Both of these techniques can be used to 
      estimate the level of past radiation exposure to a population, whereas EPR can 
      provide individual dose estimates of past exposure. This paper will review each 
      of these three biodosimeters and compare their application in selected 
      epidemiological studies.
FAU - Kleinerman, R A
AU  - Kleinerman RA
AD  - Radiation Epidemiology Branch, Division of Cancer Epidemiology and Genetics, 
      National Cancer Institute, National Institutes of Health, Department of Health 
      and Human Services, Rockville, Maryland 20852, USA. kleinerr@exchange.nih.gov
FAU - Romanyukha, A A
AU  - Romanyukha AA
FAU - Schauer, D A
AU  - Schauer DA
FAU - Tucker, J D
AU  - Tucker JD
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Radiat Res
JT  - Radiation research
JID - 0401245
RN  - 0 (GYPA protein, human)
RN  - 0 (Glycophorins)
RN  - 0 (Radioisotopes)
SB  - IM
MH  - Biological Assay/*methods
MH  - Body Burden
MH  - Chromosome Painting/*methods
MH  - DNA Mutational Analysis/*methods
MH  - Electron Spin Resonance Spectroscopy/*methods
MH  - Environmental Exposure/analysis
MH  - Glycophorins/*genetics
MH  - Humans
MH  - Radiation Dosage
MH  - Radiation Monitoring/*methods
MH  - Radioisotopes/*analysis
MH  - Relative Biological Effectiveness
MH  - Retrospective Studies
MH  - Risk Assessment/methods
MH  - Risk Factors
RF  - 121
EDAT- 2006/07/01 09:00
MHDA- 2006/08/17 09:00
CRDT- 2006/07/01 09:00
PHST- 2006/07/01 09:00 [pubmed]
PHST- 2006/08/17 09:00 [medline]
PHST- 2006/07/01 09:00 [entrez]
AID - RR3273 [pii]
AID - 10.1667/RR3273.1 [doi]
PST - ppublish
SO  - Radiat Res. 2006 Jul;166(1 Pt 2):287-302. doi: 10.1667/RR3273.1.